Disclosed are Aspergillus terreus ANU-301 strain (Accession Number: KACC49929), and ANU-301 strain (Accession Number: KACC49929) and a composition for controlling bacterial plant diseases using the culture filtrate. Since the strain or culture filtrate, according to the present disclosure, enhances the stress resistance of plants, it enables sustainable agriculture to eliminate or minimize the use of chemical pesticides.

The present disclosure relates to transgenic fungal cells and methods of making the same such that the transgenic fungal cells include one or more exogenous biosynthetic gene clusters integrated into the host genome. The genes of the exogenous biosynthetic gene cluster may be operably linked to a transgenic region of an endogenous biosynthetic gene cluster that includes a native promoter to control expression of the exogenous genes.

The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5′-phosphate as a coenzyme.

The invention provides a novel class of natural red azaphilone pigments: cavernamines and their hydroxyl-derivatives; as well as the orange/yellow precursor cavernine. Additionally, methods for their production by fermentation using a fungal strain belonging to the species Aspergillus cavernicola, is provided; and further the use of the novel pigments as a colouring agent for food items and/or non-food items, and for cosmetics. The cavernamine pigments have the structure of Formula I or II, the hydroxyl-derivative of said cavernamine pigment has the structure of Formula III: Cavernine pigments having the structure of Formula IV or V are precursors f the cavernamine pigments I-III above.

The present application provides an endophytic bacterial strain with a high camptothecin yield, which is Alternaria temuissima ZCMUKL-S1, deposited as CCTCC NO: M2021189, or Alternaria citricancri ZCMUKL-S2, deposited as CCTCC NO: M2021190, or Aspergillus versicolor ZCMUKL-S3, deposited as CCTCC NO: M2021191. The endophytic bacterial strain has a high yield of camptothecin, wherein the yield of camptothecin per unit dry mycelium powder of Aspergillus versicolor ZCMUKL-S3 is as high as 116 μg/g, which is the highest yield of camptothecin produced by endophytic bacterial strains reported at present, and has potential application value for its industrial production. In addition, the endophytic bacterial strain which can produce high camptothecin provided by the present application has good genetic stability.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

A process for enzymolysis of chicken includes the follow steps: Step 1, processing chicken into small pieces, transferring the small pieces into a reaction kettle, adding water and preheating; Step 2, stirring the chicken preheated in step 1, raising a temperature to 40 to 60° C., adding protease to perform enzymolysis; and Step 3, heating an enzymatic hydrolysate obtained in step 2 to 85 to 100° C., holding the temperature, and terminating the enzymolysis reaction. The protease is a composite flavourzyme including exo-protease produced by fermentation of Aspergillus oryzae and endo-protease produced by fermentation of Bacillus subtilis.

Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-type Aspergillus niger glucose oxidase or a mutant Aspergillus niger glucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The purpose of the present invention is to provide a method for producing selenoneine that allows production of selenoneine at higher yields as compared with a conventional technology, and, therefore, enables selenoneine production on an industrial scale. This purpose can be achieved by a method for producing selenoneine, comprising the step of applying histidine and a selenium compound to a transformant that has a gene encoding an enzyme of (1) below introduced therein and that can overexpress the introduced gene, to obtain selenoneine.

(1) An enzyme that catalyzes a reaction in which hercynylselenocysteine is produced from histidine and selenocysteine in the presence of S-adenosylmethionine and iron (II).