A process for the fermentative production of a biosurfactant brings a microorganism into contact with a medium that includes a mixture of saccharides. The mixture of saccharides includes glucose and at least one further saccharide which may be fructose, isomaltose, maltose, maltulose, or panose. The microorganism and the medium are provided under conditions where the microorganism is capable of synthesizing the biosurfactant.

The present invention relates to the use of a known enzyme denominated as a Starmerella bombicola lactone esterase (Sble) to perform a transesterification and/or hydrolysis reaction. More specifically the Sble enzyme performs a transesterification and/or hydrolysis reaction on bola amphiphilic glycolipids. The invention indeed discloses that said Sble is capable to convert bola sophorolipids into lactonic and/or acidic sophorolipids and saccharides, and, that yeast strains containing a non-functional or dysfunctional Sble enzyme and/or a disabled sble gene and/or not containing the sble gene produce (acetylated) bola amphiphilic glycolipids. In addition, the invention further discloses a method to produce non-acetylated (bola) amphiphilic glycolipids via rendering acetyltransferase enzymes At1, At2 and At3 non-functional or dysfunctional in the latter yeast strains and/or by modifying strains so that they do not contain the acetyltransferase t1, t2 and t3 gene(s) and/or by strains not containing the t1, t2 and t3 gene(s). Moreover, upon rendering the glucosyltransferase B (UgtB1) non-functional or dysfunctional in the abovementioned strains and/or upon removing and/or disabling the ugtB1 gene and/or by strains not containing the ugtB1 gene these produce acetylated and/or non-acetylated bola amphiphilic glucolipids. The invention further discloses a method to produce non-acetylated glycolipids via rendering acetyltransferase enzymes At1, At2 and/or At3 non-functional or dysfunctional and/or removing and/or disabling the glycolipid acetyltransferase genes in glycolipid producing yeast strains and/or by strains not containing the t1, t2 and t3 gene(s).

This disclosure describes methods to separate solids from liquids in a production facility. A process separates components in the process stream by applying non-condensable media to create density differences and then using a mechanical device to separate the solids from the liquids based on the density difference. The process produces the liquids and solids, which may be further processed to create valuable animal feed products.

The present invention relates to a Candida utilis strain having high tripeptide-producing ability and/or alcohol lyase activity and its use.

A preparation method of an ionic rare earth leaching agent includes the following steps: (1) domestication microorganisms with rare earth activated mineral powder culture medium to obtain a microbial suspension; (2) amplifying and culturing the microbial suspension and additives to obtain the amplified culture medium; and (3) mixing the modified sesbania gum with the amplified culture medium to obtain the ionic rare earth leaching agent. The activated mineral powder is the active metal-containing mineral powder in nature, which has excellent cation exchange function after activation, and the activated mineral powder and ionic rare earth mineral powder are used as the medium components to domesticate microorganisms, so that microorganisms can survive in the above-mentioned ionic solution and improve the leaching rate of synergistic leaching ionic rare earth.

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids. polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

A preparation method of an ionic rare earth leaching agent includes the following steps: (1) domestication microorganisms with rare earth activated mineral powder culture medium to obtain a microbial suspension; (2) amplifying and culturing the microbial suspension and additives to obtain the amplified culture medium; and (3) mixing the modified sesbania gum with the amplified culture medium to obtain the ionic rare earth leaching agent. The activated mineral powder is the active metal-containing mineral powder in nature, which has excellent cation exchange function after activation, and the activated mineral powder and ionic rare earth mineral powder are used as the medium components to domesticate microorganisms, so that microorganisms can survive in the above-mentioned ionic solution and improve the leaching rate of synergistic leaching ionic rare earth.

The present disclosure relates to heterologous production of indigoidine. Provided herein are a heterologous host cell capable of expressing a polypeptide comprising at least about 70% sequence identity to any one of SEQ ID NOs: 1-5, wherein the heterologous host cell is capable of producing indigoidine; heterologous expression systems comprising the heterologous host cell; and nucleic acids encoding the polypeptide. Also provided are methods of making indigoidine, including cell-free methods, and compositions comprising indigoidine.

The present invention relates to the field of microorganisms, and discloses a strain of Candida and application thereof. The strain of Candida has a name of XHZG06-95A3, belongs to Candida palmioleophila in taxonomy, and has a preservation number of CGMCC No. 29215. The C. XHZG06-95A3 provided by the present invention has the capacity of increasing ambient pH and dissolving P components, can reduce the acidity of acidified soil and increase the available phosphorus content therein, and can adapt to a wide range of ambient temperatures and pH values. This is the first time that a strain capable of increasing ambient pH and dissolving P components has been found in the species of C. palmioleophila.

A long-chain composition has at least one long-chain alkane selected from the group consisting of C9-18 linear or branched alkanes and at least one long-chain carboxylic acid selected from the group consisting of C9-18 linear or branched, saturated or unsaturated aliphatic monocarboxylic acids. The mass ratio of the long-chain alkane to the long-chain carboxylic acid ranges from 1:1 to 40:1. The long-chain composition has a higher fermentation degree or higher substrate utilization rate and the like, when used as a starting material in the production of long-chain dibasic acids via fermentation.