Disclosed is a method for preparing (S)-1,2,3,4-tetrahydroisoquinoline-1-carboxylic acid and derivatives thereof, comprising: taking a racemate of a compound represented by Formula (I) or a racemate of a salt of the compound represented by Formula (I) as a substrate, and making a R-isomer of the compound represented by Formula (I) in the substrate react under the catalysis of oxidative dehydrogenase to generate imino acid represented by formula (II); and converting the imino acid represented by Formula (II) into an S-isomer of the compound represented by Formula (I) in the presence of pipecolic acid reductase and a coenzyme capable of supplying hydrogen anions. The process has mild reaction conditions, strong stereoselectivity, high reaction efficiency, and high conversion rate. ##STR00001##

An endophytic fungus of Ophiopogon japonicus and use thereof provided. The endophytic fungus is isolated from the living Ophiopogon japonicus plants, an Ophiopogon plant of Liliaceae, by a traditional tissue block separation method, with a category name of Fusarium falciforme OJ106, and a deposit number of CGMCC No. 40703. The methylophiopogonanone B is prepared through the PDB liquid fermentation of endophytic fungus strains. The endophytic fungus of Ophiopogon japonicus is an important microorganism for searching for new resources of the methylophiopogonanone B, and has a high application value. TABLE-US-00001 SEQIDNO.1 cgactttggtgtgcgggagggacattaccgagttatacaactcatc aaccctgtgaacatacctataacgttgcctcggcgggaacagacg gccccgtaacacgggccgcccccgccagaagaccccctaactctg tttctataatgtttcttctgagtaaacaagcaaataaattaaaac tttcaacaacggatctcttggctctggcatcgatgaaaaacgcag cgaaatgcgataagtaatgtgaattgcaaaattcagtgaatcatc gaatctttgaacgcacattgccccgacagtattctggcgggcatg cctgttcaagcgtcattacaaccctcaggcccccgggcctggcgt tggggatcggeggaagccccctgcgggcacaacgccgtcccccaa atacagtggcggtcccgccgcagcttccattgcgtagtagctaac acctcgcaactggagagcggcgcggccacgccgtaaaacacccaa cttctgaatgttgacctcgaatcaggtaggaatacccgctgaact taagcatatcaataagcggtagtta

Use of Fusarium brachygibbosum in the production of mycelial protein is provided, belonging to the technical field of microbial fermentation. It is proposed for the first time that the Fusarium brachygibbosum shows the characteristic of a high production yield of the mycelial protein that is significantly higher than that of other types of Fusarium reported so far. The mycelial protein produced by the Fusarium brachygibbosum is filamentous, flesh-colored, elastic, and toxin-free, serving as an ideal alternative protein. Therefore, the use provides a new means for industrial production of alternative proteins and a basis for the development of safe food. Moreover, the fermentation method in the use may greatly improve the production efficiency of the mycelial protein while reducing carbon emissions to protect the environment.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.