Planting corn in one or more consecutive growing seasons in the same fields causes a yield reduction (corn-on-corn yield penalty). We developed methods and inoculants comprising Penicillium bilaii, to reduce corn-on-corn yield penalty. The disclosure covers the inoculants and methods for reducing corn-on-corn yield penalty.

The present invention discloses A METHOD TO PRODUCE PROTEIN IN PENICILLIUM AMAGASAKIENSE'S SLEEPING SPORES BY TRANSFORMATION OF SSRNA. The method includes three steps of culture of Penicillium amagasakiense and collection of spores, pretreatment of Penicillium amagasakiense spores, and electroporation of Penicillium amagasakiense spores by using HDEN method. In the present invention, non-germinated spores are used as a starting material for introduction of exogenous molecules. The exogenous protein coding single stranded RNA is introduced into the resting spores of Penicillium amagasakiense by employing the HDEN electrotransformation technique to express protein. The method of this invention is simple and fast, the effect is excellent, and the transformation rate reaches more than 90%.

The present invention discloses a method for direct transformation of exogenous DNA into resting spores of Penicillium amagasakiense. The method includes three steps of culture of Penicillium amagasakiense and collection of spores, pretreatment of Penicillium amagasakiense spores, and electroporation of Penicillium amagasakiense spores by using HDEN method, to obtain Penicillium amagasakiense spores with introduction of plasmids to be transformed. In the present invention, non-germinated spores are used as a starting material for introduction of an exogenous molecule, and exogenous DNA is introduced into the resting spores of Penicillium amagasakiense by employing the HDEN electrotransformation technique, whereby the complex step of spore germination is omitted, and steps of protoplast preparation or Agrobacterium-mediated transformation in conventional methods etc. are omitted. Moreover, the transformation efficiency is high, and at least an effect of no less than 6000 positive transformants per transformation reaction system can be achieved.

A strain of highly efficient tannin-degrading bacterium SL006 from Rosa roxburghii and application thereof are disclosed in the present application, belonging to the technical field of biotechnology. The highly efficient tannin-degrading bacterium SL006 from Rosa roxburghii has a taxonomic designation of Penicillium adametzioides, and has been preserved in the China Center for Type Culture Collection (CCTCC), with a preservation number of CCTCC NO:M 20221946. The highly efficient tannin-degrading bacterium SL006 with high tannin degradation efficiency is separated, purified and screened from moldy Rosa roxburghii pomace.

Disclosed herein are compositions comprising an isolated cellulose degrading fungus. Also disclosed are culture compositions and bioreactor compositions comprising the cellulose degrading fungus. Further described herein are filtration and extraction devices comprising the cellulose degrading fungus. Still further disclosed are bioprocessing facilities for and methods for producing co-products resulting from one or more bioprocesses of the cellulose degrading fungus.

The present invention relates to fungal strains, which are a member of the genus Penicillium and have antifungal activity, and to cell-free extracts of said strains, culture media obtainable by culturing said strains and extrolites produced by said strains, all of which have fungicidal activity. The present invention further relates to compositions comprising said strains, extracts, culture media and extrolites, and their uses in the agrochemical field and the field of controlling phytopathogenic fungi in particular.

The present disclosure provides compositions comprising novel endophytes capable of promoting germination endophytes that have a symbiotic relationship with plants. The present disclosure further provides methods of improving seed vitality, biotic and abiotic stress resistance, plant health and yield under both stressed and unstressed environmental conditions, comprising inoculating a seed with the novel endophyte strains and cultivating a plant therefrom.

The present disclosure provides aqueous inoculant compositions and methods for enhancing the survival and/or stability of microbial spores in an inoculant composition. In some embodiments, aqueous inoculant compositions of the present disclosure comprise microbial spores, one or more dispersants, one or more protectants, one or more aqueous additives and a non-aqueous liquid carrier.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The present disclosure provides novel fungal strains capable of solubilizing phosphate, as well as compositions and methods of using those strains to increase the amount/concentration of soluble phosphate in a medium that comprises insoluble phosphate.