A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Methods and materials related to producing glyceric acid and downstream products are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, methods and materials for producing glycolic acid by direct fermentation from sugars are disclosed.

Disclosed herein are methods for producing interfering RNA biopesticides, such as microbial host organisms engineered to produce interfering RNA molecules which inhibit the expression of a gene in a mosquito disease vectors by RNA interference. Also disclosed herein are polynucleotides, such as expression cassettes encoding such interfering RNA molecule and facilitating integration, such as stable integration into the host organism's genome. Further disclosed herein are compositions including the disclosed nucleotide sequences and host organisms, along with methods of using the same to control mosquito populations.

A genetically modified Komagataella phaffii yeast cell for expression of a Protein or Polypeptide of Interest (POI). comprising in its genome a recombinant nucleic acid sequence encoding a POI, and a genetic modification in the open reading frame at any one or more of position 949930 on chromosome 1 (genbank LT962476.1), position 65654 on chromosome 2 (genbank LT962477.1), position 1303485 on chromosome 4 (genbank LT962479.1), position 1323758 on chromosome 1 (genbank LT962476.1) and/or position 1491140 on chromosome 4 (genbank LT962479.1).

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

The present invention relates to process of flocculation to purify recombinant proteins from a crude fermentation broth of Insulin and Insulin analogues or derivatives at a manufacturing scale in the presence of urea and Triton-X-100 at specific pH. The flocculation process is followed by at least one centrifugation and pH adjustment step for further clarification of the broth. This is further followed by filtration for final removal of any floccules present and chromatography steps to capture the pure protein. A high product recovery of more than 95% was achieved using this process.

Provided is a thermostable glucose oxidase obtained by introducing at least one pair of disulfide bonds into an amino acid sequence of a wild-type Aspergillus niger glucose oxidase or a mutant Aspergillus niger glucose oxidase. The glucose oxidase is suitable for application in the fields of food, chemical engineering, medicine, agriculture and feeds.

Provided in the present invention is a phospholipase C mutant with high enzyme activity, a polypeptide with the sequence as shown in SEQ ID NO:7, or a polypeptide derived from the phospholipase C formed by performing substitution, deletion or addition of one or a plurality of amino acids on the polypeptide of SEQ ID NO:7 while retaining the phospholipase C activity provided by SEQ ID NO:7. The phospholipase C mutant of the present invention can improve degumming efficiency and increase the yield of diacylglycerol (DAG) during degumming.

Multi-species pest control methods, which contact or subject a target pest to a yeast strain belonging to the species Pichia kluyveri and/or with a lysate, filtrate, isolate or extract thereof. The yeast strain belonging to the species Pichia kluyveri and/or the lysate, filtrate, isolate or extract thereof is incorporated into the body of the agricultural target pest.

The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.