Provided herein are variant -1,3-N-acetylglucosaminyltransferase polypeptides capable of producing lacto-n-neotetraose, yeast cells capable of producing one or more human milk oligosaccharides, and methods of making such cells. Also, provided are fermentation compositions including the disclosed genetically modified yeast cells, and related methods of producing and recovering HMOs generated by the yeast cells.

Disclosed are microbial compositions having unique combinations of microbial species which are used in plant material recycling to form a nutrient source. Also disclosed microbial inoculants which contains such a microbial composition, water, and an optional carbon source. The microbial compositions and microbial inoculants are particularly useful in recycling high lignin-content plant material. Methods of making and methods of using the microbial compositions and the microbial inoculants are also described.

The present invention generally related to a fungal cell capable of tailored triacylglycerols. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

Disclosed herein is a method for separating a protein from a microorganism comprising a cell wall. Further compositions comprising a first fraction, a second fraction or both, derived from a microorganism comprising a cell wall and comprising a protein content between 10% and 90% by weight of the fraction, are also disclosed.

The subject invention provides environmentally-friendly systems for the management of crops, soil, water and livestock. More specifically, the invention provides for a holistic approach using microbe-based and/or biosurfactant-based soil treatment compositions to address the various pain points experienced by growers and livestock producers, including those involving soil and water management.

The invention relates to a site-specific mutagenesis modified yeast dipeptidyl peptidase III. The yeast dipeptidyl peptidase III is an isolated mutant produced by making a plurality of amino acid substitutions in a wild type yeast dipeptidyl peptidase III derived from Saccharomyces cerevisiae S288c having an amino acid sequence of SEQ ID NO. 1, said amino acid substitutions comprising substitutions at positions 570, 572 and 574, so that the site-specific mutagenesis modified yeast dipeptidyl peptidase III is an enzyme having an oxidative decomposition activity on 6-methoxy-bifuran coumarin. The site-specific mutagenesis modified yeast dipeptidyl peptidase III can be used in preparation of a feed and additives thereof, and a food and additives thereof, in which 6-methoxy difuran coumarin is eliminated, and can be used in preparation of a medicament for preventing diseases induced by 6-methoxy-difuran coumarin.

In various aspects and embodiments, the invention provides microbial cells and methods for producing advanced glycosylation products from lower glycosylated intermediates. The microbial cell expresses one or more UDP-dependent glycosyl transferase enzymes in the cytoplasm, for glycosylation of the intermediates. When incubating the microbial strain with a plant extract or fraction thereof comprising the intermediates, these glycosylated intermediates are available for further glycosylation by the cell, and the advanced glycosylation products can be recovered from the media and/or microbial cells.

Antimicrobial modified defensin or defensin-like peptides, modified C-terminal fragments of a defensin or defensin-like peptides and nucleic acids encoding the same are disclosed. Compositions comprising the defensin variant peptides and methods of their use to control microbial infections of plants and vertebrate subjects as well as contamination of feedstuffs and foodstuffs are also disclosed.

Disclosed is a process for the production of a desired oligosaccharide, the process comprises providing a genetically engineered microbial cell which possesses a saccharide importer for the uptake of an intermediate oligosaccharide, and an enzyme being able to convert the intermediated oligosaccharide by transferring a monosaccharide moiety from a donor substrate to the intermediate oligosaccharide; cultivating the genetically engineered microbial cell in the presence of an intermediate oligosaccharide to generate the desired oligosaccharide; and retrieving the desired oligosaccharide.

Strains of yeast genetically engineered to produce increased amounts of non-hydroxylated collagen or hydroxylated collagen are described. An all-in-one vector including the DNA necessary to produce collagen, promotors, and hydroxylating enzymes is also described. Methods for producing non-hydroxylated or hydroxylated collagen are also provided.