The present invention relates to a method for restoring sexual reproduction between two sterile, female strains of Trichoderma reesei using a helper strain ΔMAT, said helper strain being a fertile female strain of Trichoderma reesei in which the sexual-type locus MAT has been eliminated.

The present invention includes modified and unmodified microbes, compositions comprising these microbes, and methods of using these microbes and/or compositions for enhancing plant health, plant growth plant yield and/or drought tolerance. This disclosure also provides non-naturally occurring plant varieties that are artificially infected with microbes descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The subject invention provides method of producing Trichoderma fungi on an industrial scale. In specific embodiments, the subject invention provides methods of producing both a liquid Trichoderma-based product and a solid-state Trichoderma-based product from the same starting seed culture and inoculant.

A fungal strain of the genus Trichoderma with the designation HSA12 and compositions that contain said fungal strain or spores thereof is disclosed. The fungal strain or spores thereof are promoting stabilizing plant growth, increasing the yields of crops, inoculating soil, roots and/or above-ground plant parts with the fungal strain or spores with compositions containing said fungal strain or spores thereof, to increase the efficiency of nutrient intake and to improve the stress tolerance of crops as well as improving the structure and health of the soil or for decontaminating or remediating soil or a body of water and for stabilizing or reestablishing endangered or desired wild plant populations. Also disclosed is a set of primer pairs for amplifying microsatellite loci of the genome of the fungal strain in order to determine molecular markers and to identify the fungal strain. A method for determining the fungal strain is also disclosed.

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

Provided are methods for producing food products comprising recombinant components, and compositions used in and food products produced by such methods.

Provided is an inexpensive and efficient microbiological method for producing a protein. The method for producing a protein comprises culturing a microorganism while feeding a mixture of glucose and ammonia.

A mutant strain of Trichoderma reesei has a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by SEQ ID NO: 2. A method produces a protein, the method including a step of cultivating the mutant strain.

A mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6. A method produces a cellulast, the method including a step of cultivating the mutant strain of Trichoderma reesei, the mutant strain having a mutation that eliminates or reduces a function of a polypeptide consisting of the amino acid sequence represented by any of SEQ ID Nos: 4 to 6.