The present invention relates to a Trichoderma reesei strain which is hyper-producing and which has enhanced -glucosidase activity, as well as the use of said strain.

A biological inoculant having both fertilizing and fungicidal activity is disclosed. More particularly, a bio-inoculant having the said combined effects, comprising Bradyrhizobium japonicum and specific isolates from the Trichoderma genus is disclosed. The bio-inoculant is applied to soybean crops for preventing fungi borne diseases. More particularly, the bio-inoculant of the invention is useful for protecting soybean crops against infection by Fusarium sp., Colletotrichum sp., Cercospora sp., Sclerotinia sp. and Rhizoctonia sp.

The present disclosure is generally related to modified filamentous fungal strains (cells) comprising enhanced protein productivity phenotypes, wherein such modified strains are particularly well-suited for growth in submerged cultures (e.g., large-scale production of proteins for industrial/commercial applications). Thus, certain embodiments of the disclosure are related to such variant (modified) strains of filamentous fungus derived (obtained) from parental strains comprising a gene encoding a native GEF1 protein, wherein the variant strain comprises a genetic modification which disrupts or deletes a gene encoding a native GEF1 protein, wherein the variant strain comprises an increased protein productivity phenotype (i.e., relative to the parental strain) when grown/cultivated/fermented under the same conditions.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

A use of an application mode of Trichoderma harzianum (T. harzianum) in growth and induced resistance of Nicotiana tabacum (N. tabacum) includes the following specific operation steps: S1. experimental materials: preparation of test media and bacterial solutions; S2. experimental treatments: S2.1 control treatment; S2.2 seed soaking treatment; S2.3 root irrigation treatment; and S2.4 foliar inoculation treatment; S3. test determination indexes and methods: S3.1 determination of biological traits; S3.2 determination of physiological and biochemical indexes; and S3.3 determination of disease resistance and induced resistance indexes; and S4. data processing: subjecting data to a difference significance test. Different application modes of T. harzianum all can promote the growth of N. tabacum plants and reduce the occurrence of tobacco black shank (TBS), among which a root irrigation treatment at a transplanting stage leads to an optimal effect.

A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

The present invention relates to a process for the production of a filamentous fungus whole broth enzyme composition with low viscosity, a genetically modified filamentous fungus for production of the whole broth enzyme composition, the use of such a genetically modified filamentous fungus for the production of the filamentous fungus whole broth enzyme composition with low viscosity and a filamentous fungus whole broth enzyme composition produced by such a method.

A biological seed-coating agent (SCA) including a fermentation broth obtained based on co-cultivation of Trichoderma and Bacillus sequentially inoculated, and a preparation method thereof are provided. According to a principle of synthesis biology, Trichoderma that induces disease resistance and stress resistance and Bacillus that antagonizes pathogens and promotes crop growth are inoculated in stages for co-fermentation to prepare a microbial co-culture solution. The microbial co-culture solution is mixed with diatomaceous earth and brassinolide according to a specified ratio to prepare a biological SCA in a dosage form of a powder. The biological SCA includes high contents of spores and antagonistic and growth-promoting substances.

Disclosed are microbial compositions having unique combinations of microbial species which are used in plant material recycling to form a nutrient source. Also disclosed microbial inoculants which contains such a microbial composition, water, and an optional carbon source. The microbial compositions and microbial inoculants are particularly useful in recycling high lignin-content plant material. Methods of making and methods of using the microbial compositions and the microbial inoculants are also described.

A method and modified fermentation intermediate are disclosed for the production of polyunsaturated fatty acid (PUFA). The method comprises heat sterilizing a fermentation medium comprising dextrose to produce a heat sterilized fermentation medium, wherein the heat sterilizing converts at least a portion of the dextrose to DP2+ sugars. The method comprises combining the heat sterilized fermentation medium with an enzyme capable of converting DP2+ sugars to dextrose, thereby producing a modified heat sterilized fermentation medium comprising more dextrose and less DP+ 2 sugars than without combining the medium with the enzyme. The modified heat sterilized fermentation intermediate may be placed in contact with a microorganism to produce PUFA, wherein the microorganism is capable of utilizing dextrose to produce PUFA.