The present disclosure provides a recombinant Escherichia coli strain modified by metabolic engineering means and a method for producing L-homoserine by using the same. The strain, designated as Escherichia coli having a strain number of 13-XA, is deposited in China General Microbiological Culture Collection Center (CGMCC) with an accession number of CGMCC No. 25099, dated Jun. 16, 2022. With respect to the chromosome DNA thereof, one or more genes associated with fatty acid metabolism are knocked out or attenuated, and/or a promoter is replaced for enhancement; one or more genes associated with the L-homoserine metabolic pathway are knocked out or attenuated, and/or one or more genes associated with the L-homoserine metabolic pathway are overexpressed or enhanced, and/or one or more genes associated with the L-homoserine metabolic pathway are mutated.

Disclosed is use of a Glycine max aspartate kinase/homoserine dehydrogenase family gene GmAK-HSDH. The gene GmAK-HSDH encoding a GmAK-HSDH protein has the nucleotide sequence set forth in SEQ ID NO: 1. A constructed overexpression vector pBA002-GmAK-HSDH is transformed into a recipient material JACK by cotyledonary node transformation.

The present disclosure relates to an L-threonine export protein variant, a microorganism including same, and a method for production of L-threonine using same.