Methods and materials related to producing D-lactic acid are disclosed. Specifically, isolated synthetic or natural nucleic acids, synthetic or natural polypeptides, host cells, and methods and materials for producing D-lactic acid by direct fermentation from carbon sources are disclosed, along with methods of preparing D-lactic acid polymers.

Provided is a method for improving thermal stability of a composition comprising flavin-dependent lactate dehydrogenase (LDH). The composition comprising LDH is allowed to coexist with one or more of an anion selected from the group consisting of a monocarboxylic acid, a dicarboxylic acid, a tricarboxylic acid, a tetracarboxylic acid, a polycarboxylic acid, phosphoric acid, sulfuric acid or a salt thereof; a monosaccharide; a disaccharide; a nonionic polymer; and an ionic polymer. It is possible to reduce deactivation of LDH during preparation and storage of a lactate measuring reagent, a lactate assay kit, a lactate sensor and fuel cell, reduce the amount of LDH to be used; as well as improve thermal stability, storage stability and measurement accuracy of the measuring reagent, assay kit and sensor.

A compound includes β-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof. A purity of the compound as measured through HPLC is 95% or higher. A reactivity of the compound with lactate dehydrogenase is 30 units or higher.

Provided herein are recombinant cells comprising a heterologous L-lactate dehydrogenase, and processes of preparing L-lactic acid employing such cells.

A polypeptide that modulates the activity of at least one isoform of the native tetrameric lactate dehydrogenase, and the use thereof as a medicament for the treatment of a cancer. More particularly, linear and cyclic polypeptides that inhibit the tetramerization of the lactate dehydrogenase subunits, and compositions and kits including the polypeptides.

When lactate dehydrogenase gene and/or malate/lactate dehydrogenase gene is/are introduced into a Hydrogenophilus bacterium as well as one or more of the three lactic acid-utilizing enzyme genes on the genome of the Hydrogenophilus bacterium is/are disrupted, lactic acid-producing ability is remarkably increased. The inventors of the present invention have identified the three lactic acid-utilizing enzyme genes of the Hydrogenophilus bacterium. When lactate permease gene is further introduced into the recombinant, lactic acid-producing ability is further increased. The recombinant of the present invention effectively produces lactic acid using carbon dioxide as a sole carbon source, and therefore, it is able to efficiently produce the material of biodegradable plastics, while solving global warming caused by increased emissions of carbon dioxide.

The present invention relates to the fields of industrial biotechnology, renewable raw materials and microbial production organisms. Specifically, the invention relates to a method of producing lactic acid or lactate or one or more products selected from the group consisting of polymers, polyesters and polylactic acids. Still, the present invention relates to a genetically modified fungus comprising increased specific enzyme activities, a method of preparing said genetically modified fungus, and use of said fungus for producing lactic acid, lactate or polymers.

Yeast strains and fermentation process for producing D-lactic acid and L-lactic acid are disclosed with higher titer, higher yield, shorter time, lower pH, and higher average specific productivity.

The present invention relates to a novel 3-hydroxypropionate-lactate block copolymer [P(3HP-b-LA)], and a method for preparing same, and more specifically, provides a method for preparing a 3-hydroxypropionate-lactate block copolymer, and a 3-hydroxypropionate-lactate block copolymer produced thereby, the method comprising: a first culture step in which, by using recombinant E. coli improved so as to be incapable of biosynthesizing lactic acid, P(3HP) is biosynthesized at the early stage of culturing by having glycerol as a carbon source and through 3-hydroxypropionate-generating genes and an enhanced PHA synthase; and a second culture step in which P(3HP) production is inhibited by using a carbon catabolic repression system for selectively introducing only glucose into E. coli when glycerol and glucose are supplied together as carbon sources, and in which polylactate is biosynthesized to an interrupted P(3HP) terminus by the enabling of the expression of a lactate synthase and a lactyl-CoA converting enzyme through an IPTG induction system.

Methods and materials related to producing D-lactic acid are disclosed. Specifically, isolated synthetic or natural nucleic acids, synthetic or natural polypeptides, host cells, and methods and materials for producing D-lactic acid by direct fermentation from carbon sources are disclosed, along with methods of preparing D-lactic acid polymers.