Provided is a method for biosynthesis of (R)--hydroxytetradecanoate compounds. The method comprises the following steps of: reacting -carbonyl tetradecanoate in a ketoreductase and a glucose dehydrogenase or a fusion enzyme thereof, glucose, NADP.sup.+ and a buffer solution to obtain (R)--hydroxytetradecanoate compounds. The method is an enzyme-catalyzed biosynthesis method having simple operations, conventional equipment and an environmentally friendly process, and the prepared product has high purity, high yield and a high ee value.