The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.

The present invention relates to 25 hitherto undescribed genes of B. licheniformis and gene products derived thereform and all sufficiently homologous nucleic acids and proteins thereof. They occur in five different metabolic pathways for the formation of odorous substances. The metabolic pathways in question are for the synthesis of: 1) isovalerian acid (as part of the catabolism of leucine), 2) 2-methylbutyric acid and/or isobutyric acid (as part of the catabolism of valine and/or isoleucine), 3) butanol and/or butyric acid (as part of the metabolism of butyric acid), 4) propyl acid (as part of the metabolism of propionate) and/or 5) cadaverine and/or putrescine (as parts of the catabolism of lysine and/or arginine). The identification of these genes allows biotechnological production methods to be developed that are improved to the extent that, to assist these nucleic acids, the formation of the odorous substances synthesised via these metabolic pathways can be reduced by deactivating the corresponding genes in the micro-organism used for the biotechnological production. In addition, these gene products are thus available for preparing reactions or for methods according to their respective biochemical properties.

Disclosed herein are novel methods and compositions of matter to produce 3HB in acetogens by using a(S)-3-hydroxybutyryl-CoA dehydrogenase, Hbd2, responsible for endogenous 3HB production. In conjunction with the heterologous thiolase atoB and CoA transferase ctfAB, hbd2 overexpression improves yields of 3HB on both sugar and syngas (CO/H.sub.2/CO.sub.2), outperforming previously disclosed pathways.

A genetically engineered strain of Escherichia coli Nissle 1917 (EcN) with a modified genome designed to enhance the production of short-chain fatty acids (SCFAs) is provided. The engineered genome includes the atoB gene from E. coli K-12, responsible for encoding acetyl-CoA acetyltransferase, a crt-bcd-etfA-etfB-BHBD gene cluster from E. C. butyricum that encodes enzymes involved in the synthesis of SCFAs, specifically enoyl-CoA hydratase, butyryl-CoA dehydrogenase, and electron transfer flavoproteins, and the ptb-buk gene from C. acetobutyricum, which encodes phosphotransbutyrylase and butyrate kinase. Additionally, key genes associated with competing metabolic pathwaysldhA, frdABCD, adhE, ackA, and ptaare deleted to optimize SCFA production, particularly butyrate. This strain is intended for use in therapeutic applications where enhanced SCFA production is beneficial, such as in the treatment of coronary heart disease.

The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

Described herein are methods to convert a carbohydrate-containing source to a carbonaceous product. In some aspects, the methods include contacting a first portion of the carbohydrate-containing source with a first inoculant comprising a LAB and acetogen, thereby forming a first fermentation mixture; incubating the first fermentation mixture to produce acetate; contacting a second portion of the carbohydrate-containing source with acetate and a second inoculant comprising a solventogenic Clostridia, thereby forming a second fermentation mixture which is incubated to produce the carbonaceous product. Also disclosed herein are fermentation inoculants for the conversion of a carbohydrate-containing source to a carbonaceous product, the inoculant comprising a LAB expressing an enzyme catalyzing the production of lactate from the source, a mixotrophic acetogen expressing an enzyme that catalyzes the production of acetate from lactate and formate, and a solventogenic Clostridia expressing an enzyme that catalyzes the production of the carbonaceous product from the carbohydrate source.

This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.

This document relates to using bidirectional, multi-enzymatic scaffolds to biosynthesize cannabinoids in recombinant hosts.