A molecular manufacturing process includes contacting a platform molecule with (i) a biocatalyst and (ii) a chemical catalyst under conditions suitable to produce a value-added chemical.

Ocular antimicrobial compositions and methods and method of use thereof are provided. The compositions incorporate photoactive heterojunction materials can be incorporated into a suitable carrier, alone or in combination with an enzyme capable of generating ROS upon contact with the eye for the treatment of ocular conditions. The method includes contacting the eye of a subject in need thereof, the subject has or has been diagnosed with microbial keratitis, with an effective amount of the disclosed composition. The method further includes contacting the composition in the subject's eye with near infrared radiation (NIR).

A hierarchical catalyst composition comprising a continuous or particulate macroporous scaffold in which is incorporated mesoporous aggregates of magnetic nanoparticles, wherein an enzyme is embedded in mesopores of the mesoporous aggregates of magnetic nanoparticles. Methods for synthesizing the hierarchical catalyst composition are also described. Also described are processes that use the recoverable hierarchical catalyst composition for depolymerizing lignin, remediation of water contaminated with aromatic substances, polymerizing monomers by a free-radical mechanism, epoxidation of alkenes, halogenation of phenols, inhibiting growth and function of microorganisms in a solution, and carbon dioxide conversion to methanol. Further described are methods for increasing the space time yield and/or total turnover number of a liquid-phase chemical reaction that includes magnetic particles to facilitate the chemical reaction, the method comprising subjecting the chemical reaction to a plurality of magnetic fields of selected magnetic strength, relative position in the chemical reaction, and relative motion.

Novel glucose oxidase (GOx) variants are disclosed that have the substitutions of T30V and I94V set forth in SEQ ID NO:1, and additionally at least one further amino acid substitution in the enzyme sequence in any of the positions S53; A137; A173; A332; F414 and V560. The GOx variants herein exhibit specificity for glucose and significantly reduced oxygen consumption rates and/or increased enzyme activity for electron mediators other than oxygen. Also provided are assay devices incorporating at least one of the GOx variants herein for improved blood glucose measurements.

[Problem] To provide a physiologically active peptide-loaded stable composition for injection into living bodies. [Solution] A composition containing a triblock copolymer represented by formula (I), a polyanionic polymer and a physiologically active peptide:
CNR-PEG-CNR(I) in the formula, CNR moieties are each independently a polymer segment containing a repeating unit that contains, as a part of a pendant group, a cyclic nitroxide radical bonded to a main polymer chain via a linking group that contains at least one amino group, and PEG is a segment that contains poly(ethylene glycol).

Storage-stable compositions for generating antimicrobial activity are described. The compositions comprise an enzyme that is able to convert a substrate to release hydrogen peroxide, and an unrefined natural substance, such as a honey, that includes a substrate for the enzyme. In certain embodiments, the enzyme is a purified enzyme. In other embodiments, the substrate lacks catalase activity, and the enzyme is additional to any enzyme activity able to convert the substrate to release hydrogen peroxide that may be present in the unrefined natural substance. The storage-stable compositions do not include sufficient free water to allow the enzyme to convert the substrate. Use of the compositions to treat microbial infections and wounds is described, as well as methods for their production.

Fibers for generating antimicrobial activity are described. The fibers comprise an enzyme that is able to convert a substrate to release hydrogen peroxide, and an unrefined natural substance that includes a substrate for the enzyme, wherein the enzyme is additional to any enzyme activity able to convert the substrate to release hydrogen peroxide that may be present in the unrefined natural substance. In the presence of sufficient free water, the enzyme converts the substrate to release hydrogen peroxide, which is effective against a wide range of microbes. Wound dressings and compositions comprising the fibers are also described, as is use of the fibers, wound dressings and compositions, particularly for wound healing.

An inventive method and a device for the biotechnological reduction of sugar substances in fruit educts for the purpose of obtaining low-sugar fruit products characterized by enzymatic and/or fermentative reaction processes. Said method is characterized by a closed-loop control process, by means of which the pH value in the low-sugar fruit product is adjusted to a predetermined higher value, as compared to the pH value in the fruit educt, in such a way that during the reduction of the sugar substances by at least 30% by weight to less than 40% by weight, the pH value is increased between 0.6 and 1.0 pH units; or that during the reduction of the sugar substances by at least 40% by weight to less than 50% by weight, the pH value is increased between 0.7 and 1.1 pH units; or that during the reduction of the sugar substances by at least 50% by weight to less than 65% by weight, the pH value is increased between 0.8 and 1.2 pH units; or that during the reduction of the sugar substances by at least 65% by weight to less than 80% by weight, the pH value is increased between 0.9 and 1.3 pH units; or that during the reduction of the sugar substances by at least 80% by weight, the pH value is increased between 1.0 and 1.4 pH units;
wherein the aforementioned pH values may also turn out to be higher or lower by up to 0.1 or up to 0.2 pH units; and/or
wherein, in the case of fermentatively formed sugar alcohols having a % by weight fraction of up to 3.0% by weight, the increase in the pH value with the simultaneous reduction of the sugar substances may turn out to be less by up to 0.3 pH units, as compared to a purely enzymatic process, wherein preferably both values correlate to each other, in particular, linearly.

The present invention can be used to obtain, in particular, low-sugar fruit products, such as fruit pures or fruit preparations or fruit powder or whole fruit beverages (smoothies) or fruit juices and/or vegetable juices (regardless of whether bottled undiluted as NFC juice or rediluted as fruit juice from fruit juice concentrate) or comparable fruit beverages that can be characterized as alcohol-free.

Disclosed herein are devices for detecting presence and/or amount of an analyte, such as glucose, in a urine sample when diluted in a toilet bowl containing water and methods of using same. The disclosed devices eliminate the need to handle urine samples or a device that has been contacted with a urine sample, and can be conveniently disposed of by flushing into a sewage or septic system.

The instant disclosure provides reagent compounds, and antibody and oligonucleotide reagents, for use in a variety of assays, including immunoassays and nucleic acid hybridizations. The reagent compounds comprise a bridging antigen or bridging oligonucleotide and a latent crosslinker moiety, such as a tyramide moiety. The bridging antigens are recognizable by the antibody of a corresponding antibody reagent with high affinity, and the bridging oligonucleotides are complementary to the oligonucleotide of a corresponding oligonucleotide reagent. The antibody reagents and oligonucleotide reagents also comprise a crosslinker activation agent, such as a peroxidase enzyme. Reaction of the reagent compounds with the crosslinker activation agent results in the amplification of signal in assays for target cellular markers, including cellular antigens and nucleic acids. Also provided are detectable antibodies specific for the bridging antigens, kits comprising the reagent compounds and antibody and oligonucleotide reagents, methods of signal amplification using the compounds and reagents of the disclosure, methods of preparation of the compounds and reagents, and compositions comprising the compounds and reagents.