The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The present invention relates to the field of biotechnology, and specifically relates to a PPO polypeptide tolerant to PPO-inhibiting herbicides and use thereof. The said polypeptide contains the motif LLLNYI, wherein leucine L at position 3 in the said motif is substituted with any other amino acid, or tyrosine Y at position 5 is substituted with any other amino acid. It can be used in plants, including commercial crops, to greatly improve plant resistance to PPO-inhibiting herbicides according to the herbicide resistance characteristics and herbicide selectivity, so as to control weed growth economically.

The present disclosure relates to Beta vulgaris plants having resistance to PPO herbicides and methods of producing said plants by targeted genome editing. The disclosure further relates to genetic sequences for use with targeted genome editing technologies and/or genotyping, and herbicide-resistant PPO proteins produced from genetically engineered, non-transgenic Beta vulgaris plants.

The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.

A method for treating a microbial infection involves administering an effective amount of a compound of the formula: ##STR00001##
wherein R.sub.1 is H, alkyl, aryl, heteroaryl, R.sub.2 is H, halogen, alkyl, aryl, heteroaryl, R.sub.3 is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R.sub.4 is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R.sub.5 is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R.sub.6 is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R.sub.7 is H, hydroxyl, alkoxy, alkyl, aryl, heteroaryl, amino, amino sulfonyl, acetamide, R.sub.8 is CR.sub.3, O, S, wherein R.sub.5 and R.sub.6, R.sub.7 and R.sub.6, R.sub.5 and R.sub.4, R.sub.4 and R.sub.3 can cyclize forming a 3-10 member ring comprising C, O, S, and/or N optionally substituted with one or more R.sub.3; and administering light therapy, such as a photodynamic therapy (PDT) light source.

The present invention relates to a non-transgenic sunflower plant comprising a mutated protoporphyrinogen IX oxidase (PPO) gene encoding a mutated sunflower protoporphyrinogen IX oxidase. The present invention further relates to a method of weed control at a plant cultivation site, comprising providing the plant of the present invention and applying to said site an effective amount of a PPO inhibiting herbicide. Further encompassed by the present invention is a method for producing sunflower oil.

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.