Disclosed herein is a process for preparing enantioenriched (S)-2-aminonon-8-enoic acid by amination of 2-oxonon-8-enoic acid in the presence of an enzyme and an ammonia source.

The present disclosure relates to a recombinant microorganism capable of efficiently producing L-leucine and an L-leucine production method using the same. The production method includes: (p) incubating a recombinant microorganism having an L-leucine biosynthesis pathway or a processed product of microbial cells thereof in a predetermined culture medium (X) to thereby produce L-leucine; and (q) recovering a fraction including the L-leucine from the predetermined culture medium (X). The recombinant microorganism includes, in an expressible form, a gene encoding an amino acid dehydrogenase. The amino acid dehydrogenase at least has a catalytic effect on an L-leucine production reaction in the L-leucine biosynthesis pathway.

The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.

Isolated oxidases, isolated polynucleotides encoding the oxidases, and methods of using the oxidases to produce -oxocarboxylic acid compounds or L--amino acid compounds are described.

A polynucleotide encoding an amino acid sequence represented by SEQ ID NO: 1 or 3; a protein having an amino acid sequence represented by SEQ ID NO: 1 or 3; and a method for producing an L--amino acid compound are provided. The method includes a step (1) of reacting the protein described herein with an -hydroxycarboxylic acid compound to obtain a corresponding -oxocarboxylic acid compound; and a step of reacting a protein having the ability to aminate an -oxocarboxylic acid compound and convert the same into a corresponding L--amino acid compound with the -oxocarboxylic acid compound obtained in step (1) to obtain a corresponding L--amino acid compound.

The present invention relates to a novel biocatalytic method for the production of primary and secondary amines, comprising coupled enzymatic oxidation and reduction processes simultaneously regenerating the required cofactor; making use of two enzymesi.e. an alcohol dehydrogenase and an amine dehydrogenaseoperating simultaneously in a one pot process (i.e. biocatalytic cascade). Furthermore, the overall process is redox neutral, since the hydride generated from the first oxidative step is internally recycled in the second reductive step. The invention also relates to recombinant expression systems and microorganisms procuring the required enzyme activities; and bioreactors for performing such methods.

Disclosed are a self-assembly enzyme system supplying -ketoglutarate (-KG) and application thereof in catalytic synthesis of 4-hydroxyisoleucine. In the present disclosure, glutamate oxidase catalyzes glutamate to generate -KG, and catalase-peroxidase decomposes a byproduct H.sub.2O.sub.2. An interaction between RIAD and RIDD and a covalently linked combined state can mediate higher-order structures of various self-assembly enzymes. The LGOX/KatG self-assembly system is constructed through the affinity of short peptides in vitro to eliminate H.sub.2O.sub.2 in situ, thereby eliminating the inhibitory effect of H.sub.2O.sub.2 on Fe(II)/-KG DOs, and facilitating efficient and high-yield production of 4-HIL in a one-pot cascade reaction with IDO, with a highest yield up to 95% at a substrate concentration of 100 mM.