Provided is a genetically engineered yeast cell having increased NADPH production, a method of increasing a NADPH level in a yeast cell, a method of preparing the genetically engineered yeast cell, and a method of producing lactate using the genetically engineered yeast cell.

Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene.

This disclosure relates to recombinant microbes, systems, and methods useful in the production of psilocybin and dimethyltryptarme (DMT). The disclosure relates to modifications and optimizations to genes and enzymes directly involved in the psilocybin and DMT pathway for the production of psilocybin and DMT in a host cell. The disclosure also relates to modifications and optimizations to genes and enzymes in the host cell and medium for psilocybin and DMT production in E. coli. The present disclosure also relates to a semi-synthetic method of producing psilocybin.

A C. glutamicum strain has enhanced productivity of L-lysine, and a method produces L-lysine using the C. glutamicum strain. The C. glutamicum strain expresses NAD(P)(+) transhydrogenase subunit alpha PntA and NAD(P)(+) transhydrogenase subunit beta PntB combined with increased activity of the myo-inositol transporter IolT. A method produces L-lysine in a fermentative process employing such C. glutamicum strain.

A method for the production of value added chemicals comprising: introducing carbon dioxide to a system comprising an electrochemical cell comprising a carbon dioxide reducing catalyst and an electrolyte under conditions suitable for conversion of carbon dioxide to one or more C.sub.2 compounds; introducing at least a portion of the one or more C.sub.2 compounds to a second system comprising a microbe and a media under conditions suitable for conversion of the C.sub.2 compounds to one or more C.sub.2+n compounds where n is from about 1 to about 100. A method of preparing value added chemicals comprising: a means for converting carbon dioxide to one or more C.sub.2 compounds and a microbe wherein the C.sub.2 compound is contacted with the microbe under conditions suitable for the formation of one or more C.sub.2+n compounds.

The subject invention pertains to environmentally-friendly compositions, methods and systems for the production of diesel biofuels. More specifically, the subject invention integrates electrocatalysis and microbial bioconversion to generate lipids from carbon dioxide in a way that increases energy efficiency over known biomass-derived diesel production and significantly reduces land use.