The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type Ill secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumour antigen. Methods of imparting immunity using the recombinant bacterium are also provided.

The invention provides compositions and methods related to selective inhibition of PPO11 and use for improving shelf life of a plant or parts thereof. In accordance with the invention, for example, compositions for topical application to a plant or part thereof, are provided that can reduce browning of the plant or part thereof to extend shelf life.

A genetically modified fruit-producing plant, said plant having sufficiently reduced total Polyphenol Oxidase (PPO) activity relative to a wild type of said plant to reduce browning in the fruit of said plant relative to said wild type, wherein the reduced total PPO activity results from a reduction in activity of at least two PPO isoenzymes in said plant relative to said wild type, or a cell, seed, seedling, part, tissue, cell, fruit or progeny of said plant.

The present invention relates to a recombinant bacterium expressing an antigen that is translocated to the cytosol of a host organism, and uses thereof. To this end, the present invention provides a recombinant bacterium comprising a nucleic acid encoding an antigen that is translocated to the cytosol of a host cell utilizing Type III secretion system. The recombinant bacterium is generally chosen from intracellular pathogens that reside in the phagosome and fail to induce rapid T cell activation. The translocated antigen may be a viral antigen, a bacterial antigen, or a tumor antigen. Methods of imparting immunity using the recombinant bacterium are also provided.

A fire-protecting insulation product has air-laid mineral wool fibres and a binder. The binder is the result of curing a binder composition comprising at least one hydrocolloid. The product further comprises a particulate endothermic material.

A method for producing a potato plant with suppressed browning may use CRISPR/Cas9 system. In a method of producing a genome-edited potato plant with suppressed browning, a potato protoplast is transfected with a ribonucleoprotein complex containing sgRNA which targets StPPO2 (Solanum tuberosum Polyphenol Oxidase 2) gene derived from potato. Since the method involves no insertion of a foreign gene and includes only tiny mutation that is hardly distinguishable from natural mutation, it is expected that, unlike genetically modified organism (GMO) crops which require a huge amount of cost and time for evaluating the safety and negative environmental impacts, a great deal of cost and time can be saved in the present invention.

The invention relates to a mineral wool product comprising mineral fibers bonded by a cured binder wherein the binder in its uncured state comprises at least one protein and at least one enzyme.

The invention relates to an aqueous binder composition for mineral fibers comprising at least one hydrocolloid.