The present invention relates to compositions comprising a polymeric matrix or a gel containing functional enzymes capable of re-creating under culture conditions the cell microenvironment existing in vivo. The present invention also relates to devices for cell cultures comprising such compositions, in particular hydrogel and the use thereof to control the chemical microenvironment of a cell culture or mimic physiological or pathological conditions of the in vivo cells. The compositions and the devices described herein could be also used in vitro for evaluating the therapeutic effect of a compound on a determined cell line or on primary cells.

The present disclosure is related to systems and methods of enzymatic degradation of crystallizable polymers or copolymers and PC/IPM containing crystallizable polymers or copolymers.

The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add -carotene and other types of carotenoids, or microorganisms that producing carotenoids, or mixture comprising carotenoids into fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production for 12 times than before. With the advantages of simple process and high yield, the present invention has a bright application prospect.

The present disclosure generally relates to biological platforms for the conversion of cellulosic biomass into fuels and chemicals. More specifically, the present disclosure relates to the conversion of cellulosic materials into sugar acids or their salts, which may then be used to produce commodity chemicals. In one aspect, the present disclosure relates to a recombinant host cell including: reduced activity of one or more polypeptides having P-glucosidase activity as compared to a corresponding wild type cell, where each of said one or more polypeptides are encoded by a gene that has at least 80% sequence identity to a gene.

This invention relates to a method for extracting valorized compounds from lignin by contacting lignins with an ionic liquid and/or a deep eutectic solvent and adding a catalyst and/or a biocatalyst to assist in breaking down the source material. Converting lignin into high value chemicals adds revenues for a bio-refinery and helps to improve the economic viability of biofuel production.

The present invention relates to a valuable product in the form of a flavonoid-containing phenol mixture having intense red coloration and to a method for obtaining a valuable-substance phase, more particularly a red-colored phase, from a native substance mixture.

A method of obtaining useful material from plant biomass waste. The method uses sonication and/or microwave irradiation followed by sequential incubation with mixed fungal cultures. In particular, the method involves obtaining useful material from plant biomass waste comprising the steps of: a) subjecting the biomass waste to microwave irradiation and/or sonication; b) incubating the biomass waste from step a) with one or more enzymes extracted from Basidiomycete fungi; and c) incubating the biomass waste from step b) with one or more enzymes extracted from Ascomycete fungi.

The present invention provides a novel method for improving microbial laccase production, which relates to the field of microbial fermentation. The present invention is to add -carotene and other types of carotenoids, or microorganisms that produce carotenoids, or mixtures comprising carotenoids into a fermentation system during fermentation of Pleurotus ferulae and other higher fungi. The present invention can improve the laccase production 12 times more than before, with the advantages of a simple process and high yield.

A method of producing nanobiocatalysts includes: suspending a support material in a liquid medium; functionalizing a surface of the support material by mixing the suspended support material with a functionalizer for a first predefined time at a first predefined temperature, wherein a first broth results; applying a protein directly in the first broth after the first predefined time; mixing the protein with the first broth and incubating the protein for a second predefined time at a second predefined temperature, wherein a second broth results; immobilizing the incubated protein onto the functionalized surface of the support material, wherein a third broth results; filtering the third broth; and gathering a retentate of the filtering. By directly processing the first and second broths allows for providing a continuous process and production of the nanobiocatalysts, which can be optimized regarding time, water, chemical and energy consumption. Also, problems related to thixotrophy can be minimized.

The invention relates to methods of preconditioning pretreated cellulosic material in the presence of a combination of phenol oxidizing enzyme and glucoamylase. The invention also relates to processes of producing sugars and fermentation products including a preconditioning step. Finally the invention relates to a composition suitable for preconditioning.