A method of obtaining useful material from plant biomass waste. The method uses sonication and/or microwave irradiation followed by sequential incubation with mixed fungal cultures. In particular, the method involves obtaining useful material from plant biomass waste comprising the steps of: a) subjecting the biomass waste to microwave irradiation and/or sonication; b) incubating the biomass waste from step a) with one or more enzymes extracted from Basidiomycete fungi; and c) incubating the biomass waste from step b) with one or more enzymes extracted from Ascomycete fungi.

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

An enzyme formulation includes an encapsulated fungal enzyme which is effective for degrading at least one material selected from the group consisting of hydrocarbons, vulcanized rubber, synthetic rubber, natural rubber, vulcanized polymers and perfluorinated compounds. A degradation method includes treating one of the above-mentioned materials with an encapsulated fungal enzyme to degrade the material.

Fungus with incorporated of heterologous ligninolytic enzyme to enhance effective conversion of lignocellulosic feedstocks to various chemical productions.

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

A composition comprising mesoporous aggregates of magnetic nanoparticles and free-radical producing enzyme (i.e., enzyme-bound mesoporous aggregates), wherein the mesoporous aggregates of magnetic nanoparticles have mesopores in which the free-radical-producing enzyme is embedded. Methods for synthesizing the enzyme-bound mesoporous aggregates are also described. Processes that use said enzyme-bound mesoporous aggregates for depolymerizing lignin, removing aromatic contaminants from water, and polymerizing monomers polymerizable by a free-radical reaction are also described.

The present invention relates to recombinant bacteria to which a novel lipid peroxidation system has been applied and a method for preparing a biofuel and a biopolymer using same. The artificial lipid peroxidation system of the present invention can be used to prepare ultra-high concentrations of free fatty acids through artificial lipid peroxidation without cell death, and a biofuel and a biopolymer can be prepared therefrom. In addition, the artificial lipid peroxidation system according to the present invention increases intracellular redox energy density and can thus additionally promote carbon dioxide fixation. Accordingly, it is possible to prepare increased quantities of a biofuel and a biopolymer.

The present invention discloses a complexing agent for promoting decomposition of loquat leaf litter, which belongs to the technical field of plant science. According to the present invention, the mixed solution of Pseudomonas solution, lignin peroxidase, and leucine aminopeptidase solution is applied to the loquat leaf litter, and after in-situ decomposition for four months, the mass loss rate and the decomposition constant of the loquat leaf litter are significantly increased, thereby effectively promoting the decomposition of the loquat leaf litter. The present invention first proposes that the decomposition of the loquat leaf litter is influenced by externally applying a combination agent of enzyme and bacterial solution, which is simple in operation, and promotes the decomposition of the loquat leaf litter while increasing the speed of returning organic matters to soil, thereby improving soil fertility.

A method for enhancing the degradation performance of lignin-degrading bacteria Erwinia sp. QL-Z3 and a culture medium for culturing the bacteria. The rate of degradation of an Erwinia sp. QL-Z3 strain to lignin is optimized from 14.23% before optimization to 25.01%. Under the conditions that the initial pH value of the culture medium is 8, the nitrogen source is NH.sub.4NO.sub.3, and the addition amount of lignin is 3 g/L, the activity of an LiP enzyme can be optimized to 371.00 U/L, which is 3.53 times that before optimization. When the initial pH value of the culture medium is 9.5, the nitrogen source is NH.sub.4NO.sub.3, and the concentration of lignin is 2.5 g/L, the activity of MnP and Lac enzymes can be optimized to 839.50 U/L and 219.00 U/L, respectively, which are 3.18 and 2.84 times that before optimization.