Provided herein are non-naturally occurring microbial organisms comprising a methane-oxidizing metabolic pathway. The invention additionally comprises non-naturally occurring microbial organisms comprising pathways for the production of chemicals. The invention additionally provides methods for using said organisms for the production of chemicals.

Provided herein are non-naturally occurring microbial organisms comprising a methane-oxidizing metabolic pathway. The invention additionally comprises non-naturally occurring microbial organisms comprising pathways for the production of chemicals. The invention additionally provides methods for using said organisms for the production of chemicals.

Improved soluble methane monooxygenases and soluble methane monooxygenase systems are provided.

The invention refers to methods and compositions for stabilizing and increasing the activity of enzymatic mixtures comprising GH61 (PMO or polysaccharide monooxigenase) polypeptides used for the degradation of cellulosic material during the saccharification step of biofuel production processes. This improvement is achieved by the addition of a nickel cation to said enzymatic mixtures before and/or during the saccharification step. Thus, the invention provides compositions comprising PMOs, cellulolytic enzymes and a nickel cation, as well as methods for preparing said compositions and methods for producing fermentable sugars and bioproducts, preferably bioethanol, from cellulosic biomass in which said compositions are used.

Methylotrophic microorganisms, particularly methylotrophic yeasts and more particularly Pichia pastoris, which exhibit the ability to oxidize methane to methanol. Methods of making such microorganisms and DNA constructs for making such microorganisms. Such methylotrophic microorganisms are genetically transformed to exhibit the oxidizing activity of a soluble methane monooxygenase of a methanotrophic bacterium. Such transformed methylotrophic microorganisms contain at least three methane monooxygenase hydroxylase (MMOH) protein subunits of a methanotrophic bacterium: MMOH alpha, MMOH beta and MMOH gamma and a methane monooxygenase reductase (MMOR) of a methanotrophic bacterium.

Provided are engineered organisms which can convert methane or methanol to 3-hydroxypropionate.

Methods and compositions for the oxidation of short alkanes by engineered microorganisms expressing enzymes are described, along with methods of use.

Embodiments of the invention relate generally to methods to generate microorganisms and/or microorganism cultures that exhibit the ability to produce polyhydroxyalkanoates (PHA) from carbon sources at high efficiencies. In several embodiments, preferential expression of, or preferential growth of microorganisms utilizing certain metabolic pathways, enables the high efficiency PHA production from carbon-containing gases or materials. Several embodiments relate to the microorganism cultures, and/or microorganisms isolated therefrom.

A microorganism including a foreign gene encoding a protein having a hydroxylase activity that reduces the concentration of CH.sub.nF.sub.4-n (n is an integer of 0 to 3) in a sample, as well as a composition including the microorganism or lysate thereof, and a method of reducing the concentration of CH.sub.nF.sub.4-n in a sample using the microorganism or lysate.

This disclosure relates to the engineering of phototrophic microorganisms for conversion of alkanes into higher-value products. Recombinant phototrophic organisms such as cyanobacteria can be engineered, optionally in a modular format, to express enzymes involved in converting methane to methanol, methanol to formaldehyde, formaldehyde to central metabolic pathway intermediates, and such intermediates to n-butanol.