The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of substituted or unsubstituted, linear or branched aliphatic hydrocarbons.

A composition of matter comprising genetically modified bacteria of the Bacillus genus which over-express cyclodipeptide synthase (YvmC) and/or cytochrome P450 oxidase (CypX) is disclosed. The bacteria synthesize at least twice the amount of pulcherriminic acid as compared to the amount synthesized in a non-genetically modified bacteria of a corresponding strain.

Provided are an optimized CPY4V2 gene and an application thereof. The gene relates to a polynucleotide for coding a CYP4V2 protein, and comprises a nucleotide sequence having 90% or over 90% of identity with a nucleotide sequence as shown in SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8 or SEQ ID NO: 9. Also provided is an expression vector comprising the gene. The expression vector can realize lasting and stable expression in retinal pigment epithelium of eyes, can effectively reduce the dosage and possible side effects of a gene therapy drug for treating BCD, and improves the therapeutic effect.

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.

The present invention relates to a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO: 3, or a variant thereof having an amino acid sequence having at least 95% identity thereto and having CYP450 activity. The cytochrome P450 enzyme provided herein was isolated from Streptomyces eurythermus NRRL 2539 and has a wide substrate range and high activity, and may be used to oxidate organic compounds.

The present invention relates to a genetically modified human stem cell, wherein said human stem cell comprises an exogenous nucleic acid comprising a region encoding a fusion protein comprising a mutant human cytochrome P450 2B6 protein (CYP2B6*) of SEQ ID No. 1, or a variant or fragment thereof and a NADPH-cytochrome P450 reductase protein of SEQ ID No. 2 or a variant or fragment thereof, operably linked to a promoter, said exogenous nucleic acid having been inserted into chromosome 17 of said human stem cell. The invention also relates to the use of said cell in the prevention and/or treatment of cancer and/or associated metastases, notably solid tumours, in particular hepatocellular carcinomas, and/or recurrent cancer and/or associated metastases.

The present invention provides improved P450-BM3 variants with improved activity. In some embodiments. the P450-BM3 variants exhibit improved activity over a wide range of substrates.

The present invention relates to a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO: 3, or a variant thereof having an amino acid sequence having at least 95% identity thereto and having CYP450 activity. The cytochrome P450 enzyme provided herein was isolated from Streptomyces eurythermus NRRL 2539 and has a wide substrate range and high activity, and may be used to oxidate organic compounds.

It is an object of the present invention to provide a modified oxygenase that specifically epoxidizes only the position-3 of highly unsaturated fatty acid. The present invention relates to: a polypeptide consisting of an amino acid sequence containing one or two or more amino acid substitutions selected from the group consisting of F87K/I/H, A330V, P25L and T438M, in the amino acid sequence as set forth in SEQ ID No: 2; and a polypeptide substantially identical to the aforementioned polypeptide.

This invention relates to a device that can be used is used to detect organophosphates and carbamate on surfaces including food, clothing (including as wearable pesticide detectors) and machinery.