This document describes biochemical pathways for producing 5-hydroxypentanoate methyl ester and pentanoic acid pentyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase, and a monooxygenase, as well as recombinant hosts expressing one or more of such exogenous enzymes. 5-hydroxypentanoate methyl esters and pentanoic acid pentyl esters can be enzymatically converted to glutaric acid, 5-aminopentanoate, 5-hydroxypentanoate, cadaverine, or 1,5-pentanediol.

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of (i) Enzyme E.sub.1 capable of converting the alkane to the corresponding 1-alkanol; (ii) Enzyme E.sub.2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) Enzyme E.sub.3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) Enzyme E.sub.4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) Enzyme E.sub.5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester, and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E.sub.20-E.sub.24 selected from the group consisting of: E.sub.20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.22, E.sub.21 Acyl-CoA thioesterase, of EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22, E.sub.22 Acyl-CoA:ACP transacylase, E.sub.23 Polyketide synthase, and E.sub.24 Hexanoic acid synthase

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

The present disclosure provides a method of generating electricity from a long chain hydrocarbon, said method comprising contacting the liquid non-polar substrate with a plurality of enzymes, wherein at least one enzyme is non-electric current/potential enzyme that functions as a catalyst for chemical reaction transforming a first substrate or byproduct to a second substance that can be used with an additional electric current/potential generating enzyme.

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate, or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.

The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

An enzymatic process and system are disclosed for producing homogeneous chain length poly hydroxyalkanoate monomers and/or polymers having a chain length of at least eight carbons from a long-chain fatty acid or precursor thereof. The process also results in specific homogenous chain length polyhydroxyalkanoate monomers and/or polymers. The long-chain fatty acid or precursor thereof can be contacted in vitro with an enzyme or a mixture of enzymes to produce the homogeneous chain length polyhydroxyalkanoate.

An enzymatic process and system are disclosed for producing bioplastic polymers from a thermoplastic polymer-containing post-use product. The thermoplastic polymer-containing post-use product can be pyrolyzed to obtain a pool of depolymerized alkanes. The pool of alkanes can be contacted in vitro with an enzyme or a mixture of enzymes to produce a bioplastic polymer.