The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below: (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; and (c) both of the genes (a) and (b) above.

A bacterial strain comprising one or more vectors encoding a) one or more enzymes to produce one or more terpene precursors; and b) a fungal terpene synthase (FTPS). The present invention also relates to a method of producing a terpenoid comprising a) culturing the bacterial strain described herein in an expression medium; and b) isolating the terpenoid from said expression medium.

The present invention relates to a recombinant Deinococcus bacterium exhibiting enhanced 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DXP) pathway, and its use for producing terpene or terpenoid compounds.

The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

The present invention relates to a recombinant Deinococcus bacterium exhibiting enhanced 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DXP) pathway, and its use for producing terpene or terpenoid compounds.

A mutant carotenoidogenic bacterium, comprising any of genes (a)-(c) below: (a) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 225th amino acid residue in the amino acid sequence of 1-deoxy-D-xylulose 5-phosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; (b) a gene encoding a protein comprising a mutant amino acid sequence in which at least the 305th amino acid residue in the amino acid sequence of decaprenyl diphosphate synthase of a carotenoidogenic bacterium has been substituted with other amino acid residue; and (c) both of the genes (a) and (b) above.

Described herein are devices and methods for increasing the production of steviol glycosides, which have industrial and economic value. The steviol glycosides produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods and do not have a bitter aftertaste, making them better suited as flavor-enhancing N additives to food, pharmaceutical, and nutritional supplement products.

Described herein are devices and methods for using the same to produce carotenoids. The carotenoids produced by the devices and methods disclosed herein do not require the ultra purification that is common in conventional or commercial methods. The devices and methods disclosed herein also enhance one or more physical properties of plants treated with the devices described herein.