Disclosed are transformed cells comprising one or more genetic modifications that affect the lipid content of the cell, e.g., by increasing the concentration of oleic acid in the cell relative to an unmodified cell of the same type. Also disclosed are methods for modifying the lipid content of a cell by increasing the activity of one or more proteins in the cell and/or by decreasing the activity of one or more proteins in the same cell.

DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinum, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.

Transgenic soybean seed having increased total fatty acid content of at least 10% and altered fatty acid profiles when compared to the total fatty acid content of non-transgenic, null segregant soybean seed are described. DGAT genes from oleaginous organisms are used to achieve the increase in seed storage lipids.

Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chiarella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

Disclosed is a diacylglycerol acyltransferase 1, a recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1, and application thereof in production of triacylglycerol. The diacylglycerol acyltransferase 1 of the invention has a function of catalyzing synthesis of triacylglycerol. After the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 of the invention is subjected to induction culture for 48 h, the content of total fatty acid and triacylglycerol in the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 can be respectively increased by 1.94 folds and 12.09 folds as compared with those of Saccharomyces cerevisiae without the recombinant diacylglycerol acyltransferase 1. The instant invention provides a method for improving the ability of microorganisms to produce polyunsaturated fatty acids (PUFAs) by means of genetic engineering.

Aspects of the disclosure relate to biosynthesis of cannabinoids and cannabinoid precursors in recombinant cells and in vitro.

The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.

The present invention relates to the technical field of gene engineering and particularly relates to a method for constructing non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield, recombinant strain constructed by method, and application of recombinant strain. According to the present invention, a diacylglycerol acyltransferase gene (DGAT) is overexpressed in Mucor circinelloides WJ11 by a homologous recombination technology, and exogenous oil is added for fermentation, such that the non-de novo synthesized Mucor circinelloides recombinant strain with high lipid yield is constructed. Compared with the control strain Mc2075, the fat yield of the Mucor circinelloides is increased; and when the diacylglycerol acyltransferase (DGAT) is transformed into the uracil defective type of Mucor circinelloides WJ11, the fatty acid composition changes after fermentation, and the lipid content may reach 53% of dry cell weight after the fermentation condition is optimized.