The present invention generally related to a fungal cell capable of tailored triacylglycerols. The fungal cell comprises at least one modification to the endogenous fatty acid metabolism.

The present invention relates to the field of fungal production fatty alcohols. More specifically, the present invention relates to genetically modified host cells, nucleic acid constructs and culture medium for the production of fatty alcohols in Rhodosporidium.

The present disclosure relates to use of a multigene stacking method in synthesis of nervonic acid in Brassica napus. The present disclosure provides a multigene co-expression plant vector, including an initial backbone of pBWA(V)BII and multiple exogenous gene expression cassettes. The present disclosure further provides two plant vectors applicable to genetic transformation, including a three-gene co-expression plant vector and a five-gene co-expression plant vector. In the present disclosure, after the three-gene co-expression plant vector and the five-gene co-expression plant vector are separately transferred into the Brassica napus, the nervonic acid (NA) with a high content is synthesized in the Brassica napus through multigene co-expression. An NA ratio can be significantly increased combined with a higher seed oil content and a greater field seed yield. The plant vector realizes efficient synthesis of NA in oil crops, obtains NA-rich seed oil, and increases an added value of edible oil.

The technology described herein is directed to engineered chemoautotrophic bacteria and methods of producing triacylglycerides. Also described herein are systems or bioreactors comprising said engineered bacteria.

Provided is an engineered bacterium for producing nervonic acid and/or grease. The genome of the engineering bacterium is integrated with an expression cassette expressing a protein encoded by 3-ketoacyl-CoA synthase (KCS) gene and/or an exterase gene.

DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinum, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.

Disclosed are methods and compositions for increasing the triacylglycerol content of a cell by up-regulating diacylglycerol acyltransferase and down-regulating triacylglycerol lipase. In some embodiments, a DGA1 protein is expressed and a native TGL3 gene is knocked out, thereby increasing the synthesis of triacylglycerol and decreasing its consumption, respectively.

Disclosed are compositions for drilling and/or maintaining a wellbore, e.g., drilling fluids and drilling muds, comprising oleaginous yeast. The oleaginous yeast may comprise at least about 45 wt % oil. The oleaginous yeast may comprise a genetic modification that either increases the oil content of the yeast, alters the lipid composition of the yeast, and/or provides a selective advantage for the yeast, e.g., relative to an unmodified yeast of the same species.

The present invention provides for a method of engineering a plant having an increased content of a target compound in the plant's seed, the method comprising introducing into the plant a first nucleic acid construct that encodes a seed-specific promoter operatively linked to a transcription factor wherein expression of the transcription factor increases the production of the target compound. A genetically modified plant cell engineered by the method.

It is an object of the present invention to provide the eukaryotic microalgae, which have been genetically modified such that larger amounts of assimilation products produced by photosynthesis are directed to the synthesis of triglyceride (=triacylglycerol; TAG), and specifically, the present invention relates to a genetically modified strain of eukaryotic microalgae, in which a gene encoding an AGL1 protein is highly expressed, or a gene encoding an FAT1 protein and/or a gene encoding a DGAT2 protein are further highly expressed, as well as the gene encoding an AGL1 protein, wherein TAG productivity is improved in comparison to the parent strain thereof.