Patent classifications
C12Y203/01075
Production of fatty acids esters
A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.
Process for producing ethyl esters of polyunsaturated fatty acids
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
Process for producing ethyl esters of polyunsaturated fatty acids
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
Process for producing ethyl esters of polyunsaturated fatty acids
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
PROCESS FOR PRODUCING ETHYL ESTERS OF POLYUNSATURATED FATTY ACIDS
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
PROCESS FOR PRODUCING ETHYL ESTERS OF POLYUNSATURATED FATTY ACIDS
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
PROCESS FOR PRODUCING ETHYL ESTERS OF POLYUNSATURATED FATTY ACIDS
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
PROCESS FOR PRODUCING ETHYL ESTERS OF POLYUNSATURATED FATTY ACIDS
The present invention relates to a process for producing ethyl esters of polyunsaturated fatty acids, comprising transesterifying triacylglycerols in extracted plant lipid.
LIPID COMPRISING POLYUNSATURATED FATTY ACIDS
The present invention relates to extracted plant lipid, comprising fatty acids in an esterified form.
BIOTECHNOLOGICAL PRODUCTION OF omega-FUNCTIONALISED CARBOXYLIC ACIDS AND ESTERS THEREOF
There is provided a microbial cell for producing at least one omega-functionalized carboxylic acid ester from at least one alkane, wherein the cell is genetically modified to increase the expression relative to the wild type cell of (i) Enzyme E.sub.1 capable of converting the alkane to the corresponding 1-alkanol; (ii) Enzyme E.sub.2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) Enzyme E.sub.3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) Enzyme E.sub.4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) Enzyme E.sub.5 capable of converting the alkanoic acid ester of (iv) to the corresponding omega-hydroxy-alkanoic acid ester, and wherein the cell does not comprise a genetic modification that increases the expression relative to the wild type cell of at least one of the following enzymes E.sub.20-E.sub.24 selected from the group consisting of: E.sub.20 Acyl-ACP thioesterase, of EC 3.1.2.14 or EC 3.1.2.22, E.sub.21 Acyl-CoA thioesterase, of EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22, E.sub.22 Acyl-CoA:ACP transacylase, E.sub.23 Polyketide synthase, and E.sub.24 Hexanoic acid synthase