The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.

The present invention is related to an enzyme that allows efficient removal of fructan from grain and vegetable raw material. The enzyme according to the invention produces grain and vegetable material having a fructan content significantly lower compared to that of the starting material.

The present disclosure generally relates to sugar reduction in foods and, in some aspects, to enzyme-polymer conjugated particles for food and other applications. Certain aspects of the disclosure are directed to compositions for reducing sugar content and/or producing dietary fiber within food products during or after consumption (e.g., in a subject's gastrointestinal (GI) tract), while maintaining the sweetness and flavor of the sugar in food products upon consumption (e.g., in a subject's mouth). For example, in one set of embodiments, a composition may comprise a particle comprising an enzyme capable of converting a sugar into a relatively non-digestible form (e.g., a polymer), optionally an inhibitor that reversibly inhibits the enzyme from converting the sugar, and optionally an additive capable of associating with the inhibitor. The composition may be used for in situ conversion of sugars upon exposure to an environment condition (e.g., pH and/or temperature) in the GI tract.

The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.

The invention relates to a sweetened composition, in particular to a sweetened composition and a preparation method and use thereof, the sweetened composition consists of at least 0-99% mogroside V and 0-99% fructooligosaccharide, and the preparation method includes the following steps: take Momordica grosvenori extract, then perform enzymatic conversion treatment on the Momordica grosvenori extract by biological enzyme coupled membrane separation technology, convert sucrose contained in Momordica grosvenori into oligofructose under the action of fructosyltransferase, sucrase and glucose isomerase, convert low-sweetness glucose commonly existing in the Momordica grosvenori into high-sweetness fructose, and obtain the sweetened composition after recombination, concentration and sterilization. The sweetened composition product prepared by the invention improves the defects of insufficient sweetness and delayed sweetness of mogroside V, and can be used for preparation of food, beverages, dietary supplements, pharmaceuticals, and pet food.

This invention relates to a process for the production and purification of fructooligosaccharides (FOS) and the product obtained by such process having low or no residue of glucose, or fructose, or sucrose, with the FOS having a different ratio of oligomers (GF2, GF3, GF4, GF5 and GF6) and their isomers, to be used in animal and human nutrition due to their superior properties of modulating the intestinal microbiota and direct and indirect immunomodulation. The process for producing and purifying fructooligosaccharides according to the invention employs at least two microbes or yeasts, simultaneously or separately, with one of the microbes or yeasts being unable to produce extracellular invertase in a medium containing sucrose, in order to convert sucrose into fructooligosaccharides (FOS), with total consumption of glucose from the medium and evaporation of alcohol or ethanol during drying of the final product.

The present invention relates to a method for converting sucrose comprised in a liquid food product into at least one fructooligosaccharide (FOS), the method comprising contacting said liquid food product with an Aspergillus niger fructosyltransferase under conditions allowing enzymatic conversion of sucrose to at least one FOS by said fructosyltransferase, wherein said contacting is performed at least until the sucrose concentration in said liquid food product is at most 15 g/L; and to compositions, uses, and products related thereto.

The present invention provides nucleic acids, vectors, host cells and methods for production of fructosyltransferase from Aspergillus japonicus. The invention represents an advancement in the field of genetic engineering and provides methods for obtaining high yield of a novel recombinant fructosyltransferase encoded by ft gene of Aspergillus japonicus as a secreted protein.