The present disclosure provides engineered carboxyesterase enzymes that have the ability to catalyze amide bond formation. Also provided are polynucleotides encoding the carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes to make commercially valuable amides. Also provided are amides that are made using the engineered carboxyesterase enzymes.

The present invention is directed to pharmaceutical formulations of therapeutic proteins that comprise one or more polyethoxylated fatty alcohol (PFA) surfactants that are resistant to lipase mediated degradation. The present invention is also directed to methods of reducing aggregate and/or particulate formation in pharmaceutical formulations of therapeutic proteins and methods of maintaining a stable surfactant level in pharmaceutical formulations of therapeutic proteins.

The present invention relates to a process for enzymatic (trans)esterification/esterification of free fatty acids and glycerides. In particular, the invention relates to this process using a drying operation for water removal from enzyme reaction mixture continuously or by separating the glycerol phase from the reaction mixture, then drying the glycerol phase and recirculating to reform the reaction mixture, especially in biodiesel applications, which facilitates reduction of FFA in the biodiesel.

Disclosed herein are methods for producing tulipalin A (-methylene--butyrolactone), recombinant cells or organisms for producing tulipalin A, enzymes needed for producing tulipalin A, and nucleic acids for expression of those enzymes.

A method of making glycomonolipids; said method comprising the following steps: a) reacting glycodilipids with a hydrolase to form glycomonolipids; and b) optionally isolating the glycomonolipids.

A technique relating to a method for enzymatically producing maytansinol from an ansamitocin species, such as AP3, is provided. The method for producing maytansinol includes enzymatically producing maytansinol from an ansamitocin species with any one of various proteins.

Methods of treating a metabolic disorder in a subject are provided. Aspects of the method include enhancing extracellular Carboxylesterase 2 (CES2) activity in the subject in order to treat the subject for the metabolic disorder. Also provided are compositions for use in practicing the methods.

This invention relates to a device that can be used is used to detect organophosphates and carbamate on surfaces including food, clothing (including as wearable pesticide detectors) and machinery.