Systems, methods, and compositions relating to pretreatment and enzymatic degradation of polymeric materials comprising one or more crystallizable polymers or copolymers are generally described. Certain aspects are directed to methods comprising reacting a polymeric material comprising a crystallizable polymer or copolymer with a reactive agent to produce a pretreated polymeric material and exposing the pretreated polymeric material to a polymer-degrading enzyme. In some embodiments, the reactive agent induces chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some embodiments, the reactive agent induces chain scissions followed by chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some cases, the methods further comprise a thermal annealing step following the step of reacting the polymeric material comprising the crystallizable polymer or copolymer with the reactive agent and prior to the step of exposing the pretreated polymeric material to the polymer-degrading enzyme. During the thermal annealing step, further chain reactions (e.g., chain scission, extension, branching, and/or cross-linking) may occur.

Systems, methods, and compositions relating to pretreatment and enzymatic degradation of polymeric materials comprising one or more crystallizable polymers or copolymers are generally described. Certain aspects are directed to methods comprising reacting a polymeric material comprising a crystallizable polymer or copolymer with a reactive agent to produce a pretreated polymeric material and exposing the pretreated polymeric material to a polymer-degrading enzyme. In some embodiments, the reactive agent induces chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some embodiments, the reactive agent induces chain scissions followed by chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some cases, the methods further comprise a thermal annealing step following the step of reacting the polymeric material comprising the crystallizable polymer or copolymer with the reactive agent and prior to the step of exposing the pretreated polymeric material to the polymer-degrading enzyme. During the thermal annealing step, further chain reactions (e.g., chain scission, extension, branching, and/or cross-linking) may occur.

Detergent compositions that include a lipase variant of a parent lipase which has lipase activity and includes one or more substitutions corresponding to G23S, D27N, A40, F51I,L, E56R, D57N, V60E,K, K98I, N101D, R118, G163S, T231R, N233R, Y220F, T244E, and P256T using SEQ ID NO: 2 for numbering. Water-soluble unit dose articles including water-soluble film and a detergent composition including lipase variants. Methods of cleaning and/or treatment of surfaces using such compositions.

An isolated chimeric recombinant protein has cutinase activity. The protein includes a cutinase catalytic domain and a polymer binding domain operably linked by a proline/threonine-rich linker domain. The proline/threonine-rich linker domain includes at least 50% proline or threonine residues over a stretch of 15 to 55 consecutive amino acids.

The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to cleaning compositions comprising at least one lipolytic enzyme having polyesterase activity and at least one cellulase. The cleaning compositions described herein are suitable for use in cleaning processes and include detergent compositions, such as laundry detergent compositions, in particular liquid laundry detergent compositions. The present invention further relates to a method for cleaning textiles and to the use of the cleaning composition according to the invention for removing stains as well as for reducing pilling effects and/or increasing the anti-graying effect of the cleaning composition. Furthermore, the invention is directed to the use of at least one lipolytic enzyme having polyesterase activity and at least one cellulase for synergistically improving the anti-pilling performance and/or anti-graying performance of a cleaning composition comprising lipolytic enzyme having polyesterase activity and cellulase.

Systems, methods, and compositions relating to pretreatment and enzymatic degradation of polymeric materials comprising one or more crystallizable polymers or copolymers are generally described. Certain aspects are directed to methods comprising reacting a polymeric material comprising a crystallizable polymer or copolymer with a reactive agent to produce a pretreated polymeric material and exposing the pretreated polymeric material to a polymer-degrading enzyme. In some embodiments, the reactive agent induces chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some embodiments, the reactive agent induces chain scissions followed by chain extension, branching, and/or cross-linking of the crystallizable polymer or copolymer. In some cases, the methods further comprise a thermal annealing step following the step of reacting the polymeric material comprising the crystallizable polymer or copolymer with the reactive agent and prior to the step of exposing the pretreated polymeric material to the polymer-degrading enzyme. During the thermal annealing step, further chain reactions (e.g., chain scission, extension, branching, and/or cross-linking) may occur.

The disclosure provides constructs comprising a first fusion protein, a second fusion protein, and a linker, wherein the first fusion protein and the second fusion protein each include an affinity reagent and a reactive enzyme, and the linker includes a first and second functional groups specific for irreversibly inhibiting the first and second fusion protein reactive enzymes. The disclosure further provides a method including (a) contacting a first fusion protein including an affinity reagent and a reactive enzyme with a linker including a functional group specific for irreversibly inhibiting the first fusion protein reactive enzyme thereby coupling the first fusion protein and the linker, and (b) contacting a second fusion protein including an affinity reagent and a reactive enzyme with the linker, the linker including a functional group specific for irreversibly inhibiting the second fusion protein reactive enzyme thereby coupling the second fusion protein and the linker.

The present invention relates to novel esterase, more particularly to esterase variants having improved thermostability compared to the esterase of SEQ ID N.sup.o 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.

The present disclosure relates generally to a polypeptide having esterase activity, more specifically, to a polypeptide comprising an amino acid sequence that has at least 80% identity to amino acids 2-257 of SEQ ID NO: 1.