A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes support systems to permit arrangement of cooling media around a biological sample without potentially damaging direct contact between the cooling media and the biological sample. Systems and methods disclosed herein also include specific configurations, types, and performance characteristics of cooling media such as phase change materials to allow for extended hypothermic storage and transport.

Methods for cooling organs. The methods include using a layered organ support surface with a temperature sensor. The organ support surface can have a cushion layer, a core layer, and an insulating layer. The insulating layer can insulate the cooling media from the organ.

A method for hyper-fast cooling of samples is described. The samples are secured in a sample container, then the sample container inserted into a cryogenic cooling chamber through an opening of the cooling chamber. The sample container is placed some distance away from a cooling head, but within range of a continuous stream of pressurized liquid coolant emitted by the cooling head, where, the sample is hyper-cooled by spraying the sample container with a continuous stream of the pressurized liquid coolant.

A normothermic machine perfusion method for a pancreas is provided. First, a perfusate contained in a venous reservoir may be oxygenated such that a ratio of oxygen to carbon dioxide in the oxygenated perfusate is approximately 90%/10%. A first portion of the oxygenated perfusate may be pumped from the venous reservoir, through a dialysis filter, and back to the venous reservoir. A dialysate comprising a salt and glucose may be infused into the first portion of the oxygenated perfusate as the first portion is pumped through the dialysis filter. A concentration of glucose in the dialysate may be less than 8 mmol/L. A second portion of the oxygenated perfusate may be pumped through an arterial filter and through a pancreas graft. Venous outflow from the pancreas graft may then be pumped to the venous reservoir.

A normothermic machine perfusion method for a pancreas is provided. First, a perfusate contained in a venous reservoir may be oxygenated such that a ratio of oxygen to carbon dioxide in the oxygenated perfusate is approximately 90%/10%. A first portion of the oxygenated perfusate may be pumped from the venous reservoir, through a dialysis filter, and back to the venous reservoir. A dialysate comprising a salt and glucose may be infused into the first portion of the oxygenated perfusate as the first portion is pumped through the dialysis filter. A concentration of glucose in the dialysate may be less than 8 mmol/L. A second portion of the oxygenated perfusate may be pumped through an arterial filter and through a pancreas graft. Venous outflow from the pancreas graft may then be pumped to the venous reservoir.

The disclosure provides processes for temperature and pressure controlled cryopreservation of samples by using isochoric systems.

The disclosure provides processes for temperature and pressure controlled cryopreservation of samples by using isochoric systems.

Systems and methods herein generally relate to prolonging viability of bodily tissue, especially an organ, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. The systems and methods herein can include an electronic pump that pumps gas into an organ and a mechanical pressure regulator to release gas based on organ pressure.

Systems and methods herein generally relate to prolonging viability of bodily tissue, especially an organ, by adjusting pressure as needed to maintain a constant pressure within the organ even during external pressure fluctuations due, for example, to transportation of the organ in an airplane. The systems and methods herein can include an electronic pump that pumps gas into an organ and a mechanical pressure regulator to release gas based on organ pressure.

A method of making infused bone fibers employs the following steps: cutting or shaving whole bone into bone fibers, washing the bone fibers, demineralizing or decalcifying at least partially the whole bone or bone fibers and infusing the bone fibers with a supernatant of biologic material or a polyampholyte cryoprotectant or a combination of both to create infused bone fibers. The step of infusing includes exposing the bone fibers to a negative pressure or vacuum to draw the supernatant and/or the polyampholyte cryoprotectant into the bone fibers, or alternatively, exposing the demineralized whole bone to a positive pressure to drive the supernatant and/or the polyampholyte cryoprotectant into the bone. The resultant method creates an infused bone grafting composition having bone fibers taken from whole bone, demineralized or decalcified at least partially and infused with one or more of a supernatant of biologic material or a polyampholyte cryoprotectant or both.