A process for manufacturing a demineralised milk protein composition (MPC2), comprising (i) providing a milk protein composition (MPC); (ii) electrodialysis of the milk protein composition (MPC) on an electrodialyser (5, 200), comprising unit cells (15, 215) comprising three compartments (20, 26, 30; 220, 226, 230) and configured to substitute at least one cation by at least one hydrogen ion H.sup.+ in the milk protein composition (MPC) to obtain an at least partially demineralised and acidified milk protein composition (MPC1); (iii) electrodialysis of the milk protein composition (MPC1) obtained in step (ii); and (iv) recovering the demineralised milk protein composition (MPC2).

A process for manufacturing a demineralised milk protein composition (MPC2), comprising (i) providing a milk protein composition (MPC); (ii) electrodialysis of the milk protein composition (MPC) on an electrodialyser (5, 200), comprising unit cells (15, 215) comprising three compartments (20, 26, 30; 220, 226, 230) and configured to substitute at least one cation by at least one hydrogen ion H.sup.+ in the milk protein composition (MPC) to obtain an at least partially demineralised and acidified milk protein composition (MPC1); (iii) electrodialysis of the milk protein composition (MPC1) obtained in step (ii); and (iv) recovering the demineralised milk protein composition (MPC2).

A method of preparing a ginger water composition containing a ginger extract according to one embodiment of the present invention includes obtaining a ginger extract from a ginger root; mixing an organic solvent with the ginger extract; providing ultrasonic waves and obtaining a first solution; mixing water with the first solution; supplying heat and obtaining a second solution; and filtering the second solution.

A method of preparing a ginger water composition containing a ginger extract according to one embodiment of the present invention includes obtaining a ginger extract from a ginger root; mixing an organic solvent with the ginger extract; providing ultrasonic waves and obtaining a first solution; mixing water with the first solution; supplying heat and obtaining a second solution; and filtering the second solution.

The present invention provides a process for preparation of highly purified mogrosides mixture from low purity mogrosides mixture. The process comprises providing a mixture of low purity mogrosides, dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to form an initial solution of mogrosides, passing the initial solution through a column system, wherein the column system comprises a plurality of columns, and each column is packed with a sorbent having different affinities to impurities and mogrosides so that one or more columns retains more mogrosides than other columns, washing the columns to remove impurities with an acidic aqueous solution, a basic aqueous solution, and an aqueous alcoholic solution successively, eluting the columns with an aqueous alcohol solution that contains higher alcohol content than the aqueous alcohol solution used in the washing step, wherein the eluate from the columns with high content of mogrosides are combined, and drying the combined eluate to obtain high purity mogrosides with the content of the total mogrosides are more than 70% (w/w). The present invention also provides a sweetener mixture and product comprising high purity mogrosides.

The present invention provides a process for preparation of highly purified mogrosides mixture from low purity mogrosides mixture. The process comprises providing a mixture of low purity mogrosides, dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to form an initial solution of mogrosides, passing the initial solution through a column system, wherein the column system comprises a plurality of columns, and each column is packed with a sorbent having different affinities to impurities and mogrosides so that one or more columns retains more mogrosides than other columns, washing the columns to remove impurities with an acidic aqueous solution, a basic aqueous solution, and an aqueous alcoholic solution successively, eluting the columns with an aqueous alcohol solution that contains higher alcohol content than the aqueous alcohol solution used in the washing step, wherein the eluate from the columns with high content of mogrosides are combined, and drying the combined eluate to obtain high purity mogrosides with the content of the total mogrosides are more than 70% (w/w). The present invention also provides a sweetener mixture and product comprising high purity mogrosides.

A method for treating inflammation, cancer, neurodegenerative diseases, diabetes, cardiovascular diseases, vascular diseases, cutaneous diseases, in a subject in need thereof. The method includes administering to a subject in need thereof an effective amount of a powdered food composition, obtained from lemon containing between 1% and 30% (w/w) of eriocitrin, in a dose of eriocitrin between 5 and 35 mg per day.

A method for treating inflammation, cancer, neurodegenerative diseases, diabetes, cardiovascular diseases, vascular diseases, cutaneous diseases, in a subject in need thereof. The method includes administering to a subject in need thereof an effective amount of a powdered food composition, obtained from lemon containing between 1% and 30% (w/w) of eriocitrin, in a dose of eriocitrin between 5 and 35 mg per day.

The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.

The invention relates to a method for obtaining a protein from a plant material, wherein the method comprises the steps of i) mechanically disrupting the plant cells to obtain a plant juice in the presence of a reducing agent, ii) treating the plant juice to cause aggregation of chloroplast membranes, iii) removing the aggregated chloroplast membranes by precipitation and/or microfiltration, iv) subjecting the plant juice to ultrafiltration, and v) subjecting the soluble plant protein concentrate to hydrophobic column adsorption to remove residual chlorophyll, phenolic compounds and off-odors in a single column passage. The present invention also pertains to an apparatus and system for plant protein isolation based on this method. The isolated proteins can be economically obtained in batch scale and in large scale. Further, the invention is directed to a protein obtained by the method of the invention, a food product comprising thereof, and a use thereof.