[Problem] To provide a composition for hemostasis that can be applied uniformly to a bleeding site and exerts a high hemostatic effect. [Solution] Provided is a composition to be applied to the subject as a spray, wherein the composition is characterized in that the spray is for hemostasis, and the composition includes a self-assembling peptide, the self-assembling peptide gelling due to self-assembly when the composition is applied to the bleeding site of the subject, and the self-assembling peptide being included in the composition in a concentration having an improved hemostatic capacity in comparison to direct application.

Provided are hydrogel-based compositions and materials for wound healing and methods of using same. The hydrogel comprises nanofibers formed from protein Q, which is a variant of the cartilage oligomeric matrix protein coiled coil (COMPcc) protein, or a protein having at least 85% homology with protein Q. The hydrogel has one or more wound healing agents distributed therein and associated with the Q fibers. The wound healing agent may be exosomes, which may be exosomes produced by cells, such as exosomes produced by multipotent stromal cells and/or one or more triterpenoids. The hydrogels may be used in treatment of wounds, such as chronic wounds.

The present invention relates to the formation of hydrogels from poly-amino acids designed from the β-aggregation-prone region of functional amyloidogenic proteins that self-assemble to form a three-dimensional nanofibril matrix. Further, these water entrapped meshwork can be modulated by various physico-chemical cues. The present invention also explores the use of the designed hydrogels as cell and tissue adhesive biomaterial for tissue engineering applications. The present invention also relates to the application of functional amyloid hydrogel including but not limited to 3D tumoroid model for anticancer drug testing, optimization of drug dosage including drug-resistant tumors, repurposing of existing drugs, and patient-derived organoid. The present invention also describes the use of functional amyloid hydrogel as a depot for controlled and sustained release of therapeutics and biologic agents encapsulated within the same. The present invention also provides a moist highly water retentive material as reservoir of wound exudates thereby promotes healing.

Provided are pharmaceutical foam compositions comprising a peptone, a peptide hydrolysate or an enzymatically-hydrolyzed protein prepared by enzymatic hydrolysis of a full-length protein; methods of preparation and uses thereof.

A method of treating a surface so that the antimicrobial activity of water-soluble antimicrobial agents is retained at the surface despite repeated washing with water is described. The method uses a dispersion of a lipidated polyanionic molecule and one or more antimicrobial agents in an aqueous carrier such as water. The treatment of the surface of the fabric of adhesive bandages and stainless steel is demonstrated using a combination of crystal violet and silver nitrate as the antimicrobial agents. The method has application in the treatment of such surfaces to control microbial growth and the development of biofilms.

A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a cross-linking reaction to form a 3-dimensional matrix of the biomaterial.

The present invention relates to a wound management composition consisting of full spectrum blend of active cannabinoids and at least one hemostatic agent including astringent-class hemostats, active-class hemostats, and active-mechanical-class hemostats. Further, the composition may also include at least chemical penetration enhancer that amplifies the absorption of cannabinoids, at least one local drug to prolong the pharmacologically active time of cannabinoids, at least one antibacterial agent, and at least one antifungal agent. Methods of delivery of the wound management composition and kits are also disclosed.

Peptide dendrimers and agents are described, which can be used for polymerising fibrinogen and as haemostatic agents. The peptide dendrimers comprise a branched core, and a plurality of fibrinogen-binding peptides separately covalently attached to the branched core. The branched core comprises: i) from two to ten multi-functional amino acid residues, wherein each fibrinogen-binding peptide is separately covalently attached to a multi-functional amino acid residue of the branched core; il) a plurality of multi-functional amino acid residues, wherein one or more fibrinogen-binding peptides are separately covalently attached to each of at least two adjacent multi-functional amino acid residues of the branched core; Hi) a plurality of multi-functional amino acid residues, wherein two or more fibrinogen-binding peptides are separately covalently attached to at least one of the multi-functional amino acid residues of the branched core; iv) a plurality of multi-functional amino acid residues, wherein two or more multi-functional amino acid residues are covalently linked through a side chain of an adjacent multi-functional amino acid residue; or y) a single multi-functional amino acid residue, and a fibrinogen-binding peptide is separately covalently attached to each functional group of the multi-functional amino acid residue, The. multi-functional amino acid residues comprise tri- or tetra-functional amino acid residues, or tri- and tetra-functional amino acid residues, or the single multi-functional amino acid residue is a tri- or tetra-functional amino acid residue.

Medical dressings include a non-woven polymeric nanofiber mat embedded within a chitosan hydrogel matrix. The dressings may be obtained by electro spinning of polymeric nanofibers and thereafter incorporating a chitosan hydrogel into interstices of the mat by vacuum or positive pressure assistance. The resulting medical dressings may be optically transparent (e.g., at least about 50% up to about 95% light transmittance), flexible, and mechanically robust. The dressings may also incorporate self-adhesion promoters to allow self-adhesion to biological tissue, e.g., ocular surfaces, and/or therapeutic agents which are capable of delivering therapeutics (e.g. stem cells, drugs and the like) to the tissue surface. The dressings are especially useful as ocular bandages for the treatment and repair of ocular wounds.

The invention provides a dry biocompatible film comprising at least one film forming material and particulate egg shell membrane (ESM), wherein said particulate ESM is distributed substantially uniformly in and/or on the film and wherein said film, or portion thereof, disintegrates upon contact with a wound or an exudate thereof. The invention further provides methods for preparing the films of the invention and the uses thereof in methods to promote the healing of wounds.