The present disclosure provides tissue products produced from extracellular tissue matrices. The tissue products can include acellular extracellular matrices including combinations of different tissue types. The combination can harness various properties of the different tissues to provide improved composite structures with desired mechanical and/or biologic properties.

Methods of treating a complex wound by administering to a complex wound in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex wound. Methods of treating a complex lower extremity ulcer by administering to a complex lower extremity ulcer in the individual a therapeutically effective amount of a fetal support tissue product to treat the complex lower extremity ulcer. Methods of reducing or preventing scar formation from granulation tissue by administering a fetal support tissue product to granulation tissue. Methods of repairing a spina bifida defect by administering to the defect in the individual a therapeutically effective amount of an umbilical cord product.

Methods of bioprinting a bio-ink construct on an internal tissue defect or a chondral defect during a minimally invasive surgery on an individual in need thereof are provided, comprising: visualizing the defect; positioning a bioprinter comprising a printhead within proximity of or in contact with the defect; and ejecting a bio-ink from the printhead onto the defect to form a bio-ink layer, thereby generating a bio-ink construct. Further provided are systems for bioprinting a bio-ink construct on an internal tissue defect during a minimally invasive surgery on an individual in need thereof, comprising a control system, an endoscope, and a bioprinter comprising a printhead.

Described herein are methods of recellularizing an organ or tissue matrix.

A composition including, (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid, is provided. Moreover, a kit which includes: a syringe, a mineral particle covered with endothelial cells and mesenchymal cells organized in 2 or more cell layers attached to the mineral particle, and hyaluronic acid, is also provided. Last, a method for filling a gap in a bone of a subject in need thereof, including contacting the gap with a composition of: (a) a mineral particle, (b) endothelial cells and mesenchymal cells, and (3) hyaluronic acid is provided.

A method of producing an acellular peripheral nerve graft comprises the steps of providing a section of peripheral nerve, primary treatment of the section of peripheral nerve comprising freezing and then thawing the section of peripheral nerve, freeze-drying the thawed section of peripheral nerve longitudinally to introduce longitudinal pores into the section of peripheral nerve, and decellularization of the section of peripheral nerve comprising contacting the freeze-dried section of peripheral nerve with detergent and enzymatic decellularization agents to provide the acellular peripheral nerve graft. 9. The acellular peripheral nerve graft typically has an average pore size of at least 40 μm and a DNA content of less than 100 ng/mg.

Described herein are compositions and method for autologous adipose tissue grafting. In one embodiment, the composition comprises a recombinant partially ordered polypeptide (Fractomer) or “Fractomer” and adipose tissue from a subject. In one aspect, the Fractomer has the general structure of [(GXGVP).sub.n-α-helix].sub.m, where X can be any amino acid except proline and α-helix is any polyalanine based α-helix having about 5 to 50 Alanine residues. In another aspect, the Fractomer has the structure [(GXGVP).sub.n-GX.sup.1(A).sub.25X.sup.1].sub.m; where X is A or V; X.sup.1 is K or D; n is an integer from 10 to 20; and m is an integer from 4 to 8.

The present invention relates to an allogeneic and heterologous adipose tissue-derived extracellular matrix scaffold, and a method for producing the same.

An adipose tissue-derived extracellular matrix scaffold according to the present invention has a composition similar to the human body, a large surface area, and an interconnected porous structure, and thus has high cell affinity and allows cells to survive for long periods.

A method of corneal implantation with cross-linking is disclosed herein. In one or more embodiments, the method includes the steps of: (i) prior to implantation, treating an implant formed from donor corneal tissue or a tissue culture grown corneal stroma with a solution of sodium dodecyl sulfate (SDS), Triton X-100, benzalkonium chloride (BAK), Igepal, genipin, 100% glycerol, or alcohol for making the implant acellular, and for killing any bacteria, viruses, or parasites prior to implantation; (ii) implanting the implant into a recipient cornea; (iii) applying laser energy to the implant so as to modify the refractive power of the implant while being monitored using a Shack-Hartmann wavefront system so as to achieve a desired refractive power for the implant; and (iv) applying a cross-linking solution and irradiating the implant to cross-link the implant to prevent an immune response to the implant and/or rejection of the implant by a patient.

A sheet structure comprising two joined extracellular matrix (ECM) tissue or sheet layers and a physiological sensor disposed therebetween; the ECM tissue being derived from a mammalian tissue source that includes small intestine submucosa (SIS), urinary bladder submucosa (UBS), stomach submucosa (SS), urinary basement membrane (UBM), liver basement membrane (LBM), amniotic membrane, mesothelial tissue, placental tissue and cardiac tissue.