A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.

A method of treating a subject having a musculoskeletal disorder includes administering to a subject's articular site in need thereof an effective amount of a hyaluronic acid (HA) composition. In one embodiment, the HA composition includes an HA derivative, wherein carboxyl functionalities of the hyaluronic acid derivative are each independently derivatized to include an N-acylurea or O-acyl isourea, or both N-acylurea and O-acyl isourea. In another embodiment, the HA composition includes a crosslinked HA gel that is prepared by reacting an uncrosslinked HA with a biscarbodiimide in the presence of pH buffer in a range of between about 4 and about 8. The composite can optionally include at least one second bioactive agent other than the HA derivative, such as a steroid.

It was found that culturing of fibroblasts in the presence of effective amounts of Tumor Necrosis Factor-alpha (TNF-α) known as a factor that induces hemorrhagic necrosis of tumor and Interleukin-4 (IL-4) known for its role in onset of allergic reactions leads to increase in the number of CD106-positive and G-CSF-positive fibroblasts.

A bioinductive patch includes a patch body and a button. The patch body has an inner space. The button is disposed in the inner space of the patch body. A method for manufacturing a bioinductive patch includes step in which a patch body having an inner space is provided. The method continues with step in which a button is disposed in the inner space of the patch body.

A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.

A bioinductive patch includes a patch body and a button. The patch body has an inner space. The button is disposed in the inner space of the patch body. A method for manufacturing a bioinductive patch includes step in which a patch body having an inner space is provided. The method continues with step in which a button is disposed in the inner space of the patch body.

A method for reattaching a detached tissue to a hard tissue includes operation in which a suture anchor having a first stitch and a second stitch is provided, wherein the first stitch is divided into a first strand and a second strand, and the second stitch is divided into a third strand and a fourth strand. The suture anchor is fixed on a hard tissue. The first strand, the second strand, the third strand and the fourth strand pass through a detached tissue. A bioinductive patch is provided, wherein the bioinductive patch includes a patch body and a button. The first strand and the third strand pass through the patch body and a first suture hole of the button, and the second strand and the fourth strand pass through the patch body and a second suture hole of the button. The second strand and the third strand are knotted to form a first strand node, and the first strand node presses the bioinductive patch and the detached tissue tightly onto the hard tissue.

A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.

In one embodiment, the present invention provides a composition, wherein the composition is a porous scaffold, wherein the pores of the scaffold are from 2 to 500 microns, the composition comprising: a) a cross-linkable protein selected from the group consisting of collagen and gelatin; b) a cross-linker which induces cross-linking of the cross-linkable protein; and c) a liquid.

Compositions containing curable recombinant human collagen, and kits comprising same, which are usable in preparing modeling material formulations for additive manufacturing (e.g., 3D bioprinting) of 3D objects are provided. Methods utilizing such modeling material formulations in additive manufacturing of 3D objects having a collagen-based material in at least a portion thereof, are also provided. The formulations feature a desired viscosity at a temperature higher than 10 C. (e.g., room temperature or 37 C.) and allow performing the additive manufacturing without cooling the system or a part thereof.