The present invention discloses a use of mitochondria to promote wound repair and/or healing. Specifically, when a certain amount of mitochondria or a composition containing a certain amount of mitochondria is administered to a wound, the effect of promoting wound repair or accelerating wound healing can be effectively achieved.

The present disclosure relates to methods and devices for the separation of blood components including separation by rapid sedimentation, including in an automated fashion.

A modular cassette and method for separating a composite fluid into at least two component parts thereof during centrifugation is provided. The modular cassette includes a fluid inlet portion, at least one fluid separation portion, at least one media chamber in fluid communication with the fluid separation portion, a fluid collection portion, at least one fluidic channel configured to form a fluid communication between at least two components of the cassette, at least one wax valve including undulating flow channel portions configured to close at least one of the fluidic channels, and at least one heating element configured to actuate the at least one wax valve.

A system and associated method for concentrating and separating components of different densities from fluid containing cells using a centrifuge includes a syringe and a syringe holder, the syringe having a proximal top with a luer port, a sidewall extending from the top forming a syringe tube, and a plunger slidably disposed inside the syringe tube, the plunger forming a sealing engagement with the sidewall, the syringe holder defining a cavity for receiving the syringe, wherein a distal end of the syringe tube is at least partially closed by the syringe holder after the plunger is placed inside the syringe tube. The method includes receiving a fluid containing cells in the syringe; placing the syringe and syringe holder into a centrifuge; exposing the syringe and syringe holder to elevated g force in the centrifuge, the syringe holder being in physical contact with the sidewall on the distal end to provide support and prevent fluid from leaking outside the syringe; removing the syringe from the centrifuge; and extracting separated layers containing cells using the luer port of the syringe as an access port.

A system and method of isolating extracellular vesicles. The method includes loading one or more of blood or bone marrow into an input port of a concentration system and centrifuging one or more of the blood or bone marrow to separate one or more of red blood cells, platelet poor plasma, or platelet rich plasma/bone marrow concentrate fractions via a centrifuge device. The method further includes pumping one or more of bone marrow/platelet rich plasma fractions and platelet poor plasma fractions into a receptacle of the concentration system and adding a concentrated aqueous two-phase solution to one or more of the bone marrow concentrate/platelet rich plasma fractions and platelet poor plasma fractions. The method also includes drawing the concentrated aqueous two-phase solution and one or more of the bone marrow concentrate/platelet rich plasma fractions or platelet poor plasma fractions back into the centrifuge device to isolate one or more of extracellular vesicles and platelet rich plasma/bone marrow concentrate fractions.

The present invention relates to a platelet rich plasma (PRP) extracting device and extracting method using the same, and more particularly, to a platelet rich plasma (PRP) extracting device capable of quickly and effectively extracting the highly concentrated PRP from centrifuged blood by a simple manipulation.

A centrifugal device includes a container having a body with a first end and a second end disposed opposite to the first end. A cap is coupled to the second end of the container, and the cap includes a top surface having at least one port configured to receive or transmit one or more of air or fluid. So configured, the container is moveable between an upright position, in which a first fluid disposed in the container is centrifuged to precipitate at least one extracellular vesicle separate from the first fluid, and an inverted position in which one or more of the first fluid having at least one extracellular vesicle depleted therefrom is removed from the container and a second fluid mixed with the at least one extracellular vesicle removed is withdrawn from the container for injection.

A dual chambered syringe includes: an inner barrel defining a first inner chamber, the inner barrel having an apertured stopper at its distal end, the inner barrel being open at its proximal end; a shaft adapted to fit within the inner barrel, the shaft having a distal end which is engageable with the aperture of the stopper; a device for controlling engaging and disengaging of the distal end of the shaft with the aperture of the stopper; an outer barrel concentric with the inner barrel defining a second inner chamber, the outer barrel having a distal end for receiving and dispensing fluids and a proximal end into which the distal end of the inner barrel is insertable into the second inner chamber; the apertured stopper engages the second inner chamber of the outer barrel and selectively prevents or permits the passage of fluids between the outer barrel second chamber and the inner barrel first chamber; the inner barrel having an engageable surface on its outside surface; and, the outer barrel having operatively associated therewith an engaging device for selective engagement and disengagement with the engageable surface on the inner barrel.

Systems and methods are provided for separating blood into two or more components. A blood separation system includes a blood separation device and a fluid flow circuit configured to be mounted to the blood separation device. The blood separation device includes a centrifugal separator and a spinning membrane separator drive unit incorporated into a common case, which allows for fluid separation by two different methods. Depending on the separation procedure to be carried out, the fluid flow circuit paired with the blood separation device may include only one separation chamber configured to be mounted to the centrifugal separator or spinning membrane separator drive unit or two separation chambers, with one being mounted to the centrifugal separator and the other to the spinning membrane separator drive unit. The system may be used to separate and collect any combination of red blood cells, plasma, and platelets.

The invention relates to a system for platelet release from a fluid comprising in particular megakaryocytic cells comprising cytoplasmic extensions.

The system allows to reproduce the pipetting process performed manually by means of a pipette, thereby permitting a continuous and automatic release of platelets.

This is performed on a large scale since the geometry of the system according to the invention allows to treat large volumes of fluid with a high flow rate (of the order of a plurality of liters per hour), thereby making it particularly suitable for use on an industrial scale.

The invention also relates to a method for platelet release from a fluid comprising in particular megakaryocytic cells comprising cytoplasmic extensions, said method being implemented by means of the abovementioned system.