The present invention relates to methods for treating cannabis biomass in order to isolate tetrahydrocannabinolic acid (THCA). In another aspect, the present invention relates to a process that involves purification followed by extraction of cannabis biomass to isolate and improve yields of high-purity crystalline extracts.

A method of making CBD concentrate or CBD Isolate comprises (a) milling a raw material; (b) contacting the milled raw material with an extraction solvent and separating a solid waste material to form a filtered extract; (c) concentrating the filtered extract; (d) washing the concentrated extract to form an organic phase and an aqueous phase; (e) separating the aqueous phase from the organic phase to form a washed extract; (f) removing an organic solvent from the washed extract to form a concentrated washed extract; (g) decarboxylating the concentrated washed extract; (h) vacuum distilling the decarboxylated extract to form a distillate; (i) dewaxing the distillate to form a post-dewax filtrate; (j) applying a vacuum to the post-dewax filtrate to form a post-dewax concentrate; (k) degassing the post-dewax concentrate; and (l) vacuum distilling the degassed concentrate to form a CBD concentrate.

Methods of isolating phenols from phenol-containing media. The methods include combining a phospholipid-containing composition with the phenol-containing medium to generate a combined medium, incubating the combined medium to precipitate phenols in the combined medium and thereby form a phenol precipitate phase and a phenol-depleted phase, and separating the phenol precipitate phase and the phenol-depleted phase. The methods can further include extracting phenols from the separated phenol precipitate phase. The extracting can include mixing the separated phenol precipitate phase with an extraction solvent to solubilize in the extraction solvent at least a portion of the phenols originally present in the phenol precipitate phase.

A powdered, water-soluble Full Spectrum Hemp Oil is formed using organic materials and natural products to form a non-GMO, fast acting, whole plant hemp extract without harsh chemicals such as hexane. The Full Spectrum Hemp Oil may be extracted using CO2 concurrent gas extraction to avoid use of chemical additives.

A method of isomerizing Δ9-tetrahydrocannabinol (“Δ9-THC”) to Δ10-tetrahydrocannabinol (“Δ10-THC”). The method includes the steps of: extracting Δ9-THC from cannabis biomass, which optionally contains one or more of the components found in fire retardant such as PHOS-CHEK®; dewaxing of crude extracts by winterization; pH-adjusting extracts by washing the extracts in heptane solution with aqueous solutions of: citric acid, sodium bicarbonate, and brine; isomerizing Δ9-THC to Δ10-THC by exposure to suitable conditions and in the presence of a catalyst based on the components of fire retardant; vacuum distillation of Δ10-THC at a predetermined temperature range and vacuum level; collecting the distillate and redistilling it up to three times to acquire distillate containing less than 60% Δ10-THC; and purification of the MO-THC to a purity of 99% or greater by crystallization from n-pentane solution.

Methods for increasing cannabinoid production in growing cannabis plants, and for lowering the hydrophobicity of soil are described. Foliar application of compositions comprising cannabinoids extracted from Cannabis sativa hemp to the growing plants and/or drenching the soil surrounding the growing cannabis plants therewith have been demonstrated to produce significant changes in cannabinoid production, thereby generating an increase in the marketable portion of the crop. Additionally, application of these compositions to the soil reduces soil hydrophobicity.

A method and system of using a supercritical fluid are disclosed. Exemplary methods include using temperature and pressure variation (e.g., above or below supercritical conditions) to remove material from a substrate to form separated and to cause movement of fluid within the system.

The invention provides compositions and methods for the breeding, production, processing and use of specialty Cannabis.

A method for performing a multi-phase extraction of plant biomass to obtain active substances is provided. The method uses a novel freeze-dry extraction technique to obtain a volatile terpene fraction from frozen plant material. In particular, the method disclosed provides terpene extracts with compositions that strongly resemble that of the parent plant material.

This disclosure provides methods and systems for the supercritical fluid extraction of cannabinoids from cannabis. The supercritical fluid extraction of cannabinoids is performed with carbon dioxide (CO.sub.2) balanced with one or more hydrocarbons, such as propane, propene, and propadiene. As demonstrated, the extraction can be carried out at maximum efficiency and energy savings while keeping the wax formation at minimum by lowering temperature. The methods and systems disclosed herein reduce the production time and safety/environmental hazards and are suitable for proper and safe extraction in non-GMP and GMP environments.