The present invention refers to a method for the separation of peptide aggregates and fragments from solutions containing target peptide.

A method for separating at least two metals from each other in a metal refining process, the method comprising: injecting a feed solution comprising the metals into a column or flow pipe comprising a monolithic solid body having a plurality of channels; and flowing the feed solution through the plurality of channels in the monolithic solid body to separate the metals.

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

A method and apparatus for enabling larger injection volumes and for reducing extra column effects in chromatographic separations using focusing pre-columns placed upstream of the analytical, or preparative, column with applications in any chromatographic system where the requirement is that the focusing pre-column, placed upstream of the analytical column, allows larger injection volumes to be utilized and, by enabling efficient focusing of solutes onto the analytical column, results in a significant reduction of band broadening due to extra-column effects which act upstream of the analytical column.

A method for producing lead-212 of very high radiological purity from an aqueous solution comprising thorium-228 and daughters thereof. Manufacture of radiopharmaceuticals based on lead-212, which are useful in nuclear medicine and, in particular, in targeted alpha radiation therapy for the treatment of cancers.

The present invention provides a column filler for liquid chromatography that has a great adsorption capacity, adjustable adsorption selectivity, and high shape retainability and therefore is usable for measurement of various substances and capable of achieving excellent separation performance and a high filling rate in a column when used as a column filler for liquid chromatography. Provided is a column filler for liquid chromatography including carbon-coated porous particles, the carbon-coated porous particles including porous particles each having a coating layer containing an amorphous carbon on a surface.

A sample injector configured to introduce a sample fluid into a mobile phase, wherein the mobile phase is to be driven by a mobile phase drive through a separation unit for separating compounds of the sample fluid in the mobile phase, wherein the sample injector comprises a metering device being operable for displacing fluid and for intaking a metered amount of the sample fluid into the sample injector, an injector valve being switchable for operating the sample injector selectively in a sample intake mode in which the metering device is operable to intake the sample fluid from a sample container, or a separation mode in which intaken sample fluid is driven between the mobile phase drive and the separation unit for separating the compounds, and a flow direction controller configured for defining an enabled flow direction of fluid displaced by the metering device and for defining a disabled flow direction.

Embodiments of a method of purifying a lysophosphatidylcholine (e.g., LPC-DHA and/or LPC-EPA) from a composition containing the lysophosphatidylcholine and at least one impurity, e.g., from phospholipids, free fatty acids, triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), glycerol, sterols, tocopherols, vitamin A, flavonoids, and minerals can use a continuous simulated moving bed process, a batch column chromatography method, or a single column to provide a purified composition of the lysophosphatidylcholine. The purified lysophosphatidylcholine (e.g., LPC-DHA and/or LPC-EPA) products can be used in various pharmaceutical and nutraceutical applications, e.g., for treating and/or preventing a neurological disease or disorder.

A chromatography system comprising at least two chromatography units (3) connected in parallel, wherein said at least two chromatography units (3) each comprises a convection-based chromatography material, wherein an initial difference in back pressure provided from the different chromatography units (3) is compensated dynamically during run of the system due to a change of chromatography unit properties provided during the chromatography process.

This disclosure relates to a process which involves: (a) providing an aqueous solution of a protein and a polyalkoxy fatty acyl surfactant of formula I

##STR00001##

wherein R.sup.1—C(═O) is a fatty acyl group, R.sup.2 is H or a substituted or unsubstituted hydrocarbyl group, X.sup.1 is O or NH, X.sup.2 is O or NH, n is 0 or an integer of 1-5, R.sup.3 is a polymeric group comprising polymerized units of formula II and III,

##STR00002##

and (b) subjecting the aqueous solution to a bioprocess.