The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region.

Systems and methods for performing simultaneous nucleic acid amplification and detection. The systems and methods comprise methods for managing a plurality of protocols in conjunction with directing a sensor array across each of a plurality of reaction chambers. In certain embodiments, the protocols comprise thermocycling profiles and the methods may introduce offsets and duration extensions into the thermocycling profiles to achieve more efficient detection behavior.

A heating hood according to the disclosure comprises a spherically formed heat transfer region, in particular for receiving at least partially spherical objects; a frame device, wherein position of the heat transfer region is at least partially predefined by the frame device; and a heating device arranged between the heat transfer region and the frame device. A slit for guiding air is formed at least sectionally between the frame device and the heating device, wherein air can be introduced into the slit on a first side of the frame device, and wherein the introduced air can be diverted from the slit on another side, which is spaced apart in a longitudinal direction of the frame device.

An incubation system includes an actuator, a platform, an incubation lid, and a dispenser. The actuator includes a motion disc and a shaft connected to the motion disc. The shaft extends away from the motion disc. The platform is connected to the shaft of the actuator in a manner allowing movement transmission. The platform has a through hole and a thermal conductive plate. One end of the through hole is sealed by the thermal conductive plate. The incubation lid is movably disposed over the platform. The platform is thermal insulating. The incubation lid has an opening allowing fluid communication, and the dispenser suspends over the thermal conductive plate of the platform.

Systems for performing cellular analysis and related devices for conditioning environments adjacent chips in such systems. A device for conditioning an environment adjacent a chip in a system for performing cellular analysis, the device includes a cover for being disposed adjacent the chip and comprising a planar body having a top surface, a bottom surface, and an outer edge surface. The cover includes a central opening extending between the top surface and the bottom surface and bounded by an inner edge surface of the cover. The cover also includes a fluid inlet extending into the body from the outer edge surface between the top surface and the bottom surface the fluid inlet arranged to accept a gas to be delivered to the central opening. The cover also includes a plurality of fluid outlets defined in the inner edge surface and in fluid communication with the fluid inlet. The plurality of fluid outlets are arranged to receive the gas from the fluid inlet and exhaust the gas into the central opening.

The present invention provides a refrigerating/heating device that efficiently refrigerates and heats while suppressing device costs. This refrigerating/heating device for efficiently heating and refrigerating is provided with: a refrigeration chamber; a Peltier-type cooler for supplying cold air to inside the refrigeration chamber; a heat radiation member for radiating Peltier heat; fans for air-cooling the heat radiation member; an exhaust duct through which waste heat from the fans and the heat radiation member passes; and an installation part to which a subject to be heated can be installed. The subject to be heated is installed in the waste heat flow path of the exhaust duct and heated.

Various embodiments of the present disclosure are directed to temperature-controlled storage. In one example embodiment, a device for temperature-controlled storage is disclosed including a plurality of receiving areas for items to be stored. At least some of the plurality of receiving areas include temperature control medium lines, temperature control medium and at least one elastic element. The temperature control medium flows through the temperature control medium lines and thereby controls the temperature of the items to be stored. The at least one elastic element secures the items to be stored.

Embodiments include a reaction vessel having a first reaction chamber filled with a first material; a first light absorbing region adhered to an interior-facing surface of the first reaction chamber; a second reaction chamber filled with a second material; a second light absorbing region adhered to an interior-facing surface of the second reaction chamber; a temperature sensor disposed within the second reaction chamber; and one or more energy sources configured to direct light at the first light absorbing region and the second light absorbing region. A processor may be employed to determine a first temperature of the first material from a second temperature of the second material measured by the temperature sensor. Methods of manufacturing such a reaction vessel are also disclosed.

An immunoblotting instrument and a control method are provided. The immunoblotting instrument comprises an immunoblotting instrument body, and an immunoblotting instrument control device, an incubation device and a liquid feeding and sucking device that are provided on the immunoblotting instrument body, and further comprises a temperature control device for controlling the incubation environment temperature of the immunoblotting membrane. By setting the temperature control device in the immunoblotting instrument to control the temperature of the reaction environment between the immunoblotting membrane and the reagent, the immunoblotting membrane can contact and react with the reagent under the same and set environmental condition, so that the reliability of the final detection results can be improved; at the same time, each functional module is associated and controlled by the control device, so that the entire immunoblotting process is automatically completed, reducing manual intervention and improving work efficiency.

A detection apparatus configured for PCR testing includes a carrier structure and a temperature detection module. The carrier structure is configured to carry a test tube, and the test tube is configured for containing a reagent and a specimen. The temperature detection module is disposed on the carrier structure and includes a heat transfer medium and a temperature sensor. The heat capacity of the heat transfer medium is approximately equal to a preset value, so as to match heat capacity of the reagent. The temperature sensor is configured to sense a temperature of the heat transfer medium. In addition, a method for detecting a temperature of the detection apparatus is also provided.