A photoelectrical device for detection of bacterial cell density includes a substrate, a driving electrode layer, an AC power source and a photoelectric conversion layer. The driving electrode layer is disposed on the substrate and includes a central electrode and a peripheral electrode pattern surrounding the central electrode. A fluid sample is disposed on the driving electrode layer. The AC power source is electrically connected to the driving electrode layer, and used to produce a non-uniform alternating electric field in the fluid sample on the driving electrode layer for driving the target bioparticles to gather up on the central electrode to form a particle cluster. The photoelectric conversion layer is used for receiving a light detecting beam after passing through the particle cluster and outputting an electric current based on the optical density of light detecting beam. The electric current changes as a concentration of the target bioparticles changes.

In a detection method, first dielectric particles each capable of being bound to a first target substance and second dielectric particles each capable of being bound to a second target substance are caused to react with a sample that contains a first target substance and a second target substance, the second dielectric particles having a different dielectrophoretic property from the first dielectric particles, a first composite particle to which the first target substance is bound is separated from the other first dielectric particle, and a second composite particle to which the second target substance is bound is separated from the other second dielectric particle by causing dielectrophoresis in the sample after the reaction, and the first target substance contained in the separated first composite particle and the second target substance contained in the second composite particle are each detected.

In one embodiment, a method for isolating and detection one or more biomarkers of interest on a dielectrophoresis device includes receiving a first biological sample containing one or more biomarkers of interest onto a dielectrophoresis (DEP) electrode array, applying a DEP force through particular electrodes of the DEP electrode array, wherein a strength, direction, and period of time of the DEP force is specific to the one or more biomarkers of interest of the first biological sample, and determining, via a digital sensor, a quantity of the one or more biomarkers of interest of the first biological sample.

A microfluidic device may, in an example, include at least one microfluidic channel, a dielectrophoresis separator to separate a plurality of cells passing within the at least one microfluidic channel, and a thermal resistor to eject at least one cell from the microfluidic device. A cassette may, in an example, include a die coupled to a substrate of the cassette, the die including at least one microfluidic channel, a dielectrophoresis separator along the microfluidic channel to separate a plurality of cells passing within the microfluidic channel, and an ejection device to eject at least one of the plurality of cells into an assay well.

A working fluid filtration and separation system that removes contaminants from a working fluid of a working machine. The system includes a vessel and an electrostatic collector mounted within the vessel which electrostatically removes contaminants from contaminated working fluid as it passes through the electrostatic collector element. The electrostatic collector element includes a plurality of concentric electrodes of different radii, a plurality of corrugated walls residing in spaces located between adjacent electrodes. An elongated center post electrode is provided that induces a voltage in at least one of the plurality of concentric electrodes. The system is configured to generate a voltage difference between each pair of adjacent concentric electrodes to electrostatically remove contaminants from the working fluid as it flows through the filtration and separation system. The system further includes a center post isolator that is configured to mount the center post isolator within the vessel and electrically insulate the center post electrode from the vessel.

An analysis device (200) analyzes a crossover frequency at which a dielectrophoretic force on dielectric particles switches from a repulsive force to an attractive force or from the attractive force to the repulsive force, comprising a flow channel (5), a pair of electrodes (22, 23), a power supply (24), an imaging unit (25) and an analyzer (26). Through the flow channel (5), a sample solution containing the dielectric particles in the dielectrophoretic liquid flows. The pair of electrodes (22, 23) are arranged in the first channel. The power supply (24) applies a frequency-modulated AC voltage to the first electrodes (22, 23). The imaging unit (25) captures an image of a movement trajectory of each of the dielectric particles flowing between the electrodes (22, 23) in the flow channel. The analyzer (26) obtains the crossover frequency of the dielectric particles based on the captured image of the movement trajectory.

A particle manipulation device includes a substrate and a microchannel included in the substrate and configured to receive a fluid including particles therein. A biasing structure is formed on the substrate adjacent to, but outside the microchannel. The biasing structure is configured to dispense radiation at a frequency to bias movement of the particles within the microchannel from outside the microchannel.

A photoelectrical device for detection of bacterial cell density includes a substrate, a driving electrode layer, an AC power source and a photoelectric conversion layer. The driving electrode layer is disposed on the substrate and includes a central electrode and a peripheral electrode pattern surrounding the central electrode. A fluid sample is disposed on the driving electrode layer. The AC power source is electrically connected to the driving electrode layer, and used to produce a non-uniform alternating electric field in the fluid sample on the driving electrode layer for driving the target bioparticles to gather up on the central electrode to form a particle cluster. The photoelectric conversion layer is used for receiving a light detecting beam after passing through the particle cluster and outputting an electric current based on the optical density of light detecting beam. The electric current changes as a concentration of the target bioparticles changes.

A light source module for microparticles sorting performed in a light-induced dielectrophoresis chip is provided, which includes a light emitting element, a control unit and a light converting unit. The light emitting element is configured to generate and emit light. The control unit is configured to generate a driving signal based on image data. The Light converting unit is coupled to the control unit, and is configured to convert the light into a light pattern based on the driving signal. A luminous exitance of the light converting unit is between 910.sup.4 lux and 1.210.sup.5 lux.

A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.