In an embodiment, the present disclosure pertains to a bioreactor. In some embodiments, the bioreactor includes an inlet and an outlet, a chamber having a wall and a cell area, and a screw drive. In some embodiments, the inlet and the outlet are in fluid communication via the chamber. In a further embodiment, the present disclosure pertains to a method of modeling peristalsis. In some embodiments, the method applying at least one of axial strain, multi-axial strain, or shear stress to a wall within a bioreactor of the present disclosure, and measuring mechanical forces applied on the wall via the screw drive.

An object of the present invention is to provide a novel approach capable of conveniently producing a large quantity of substantially uniform size cell aggregates. Provided is a method for producing cell aggregates using a cell culture bag, the cell culture bag having a lower face comprising a plurality of recesses, the method comprising the steps of: (1) adding cells and a medium to the cell culture bag, stirring the contents, and culturing the cells while applying a pressure to the cell culture bag; and (2) recovering the formed cell aggregates after the completion of culture.

A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.

Embodiments described herein generally provide for expanding cells in a cell expansion system. The cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. In further embodiments, the cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

Disclosed are a microfluidic system and method for isolating target cells or vesicles in a fluid. The system of the present invention comprises a fluid passageway having an inlet and an outlet; one or more ultra-high frequency acoustic resonator capable of generating bulk acoustic waves in the fluid passageway at a frequency of about 0.5-50 GHz; a power regulator which adjusts the power of the bulk acoustic waves generated by the ultra-high frequency resonator; and a flow rate regulating device that regulates the velocity of the solution flowing through the bulk acoustic wave region. Adjusting the power of the generated bulk acoustic waves by means of the power regulator and/or adjusting the velocity of the solution flowing through the bulk acoustic wave region by means of the flow rate regulating device allow cells or vesicles to stay in a bulk acoustic wave-affected region. The system and method of the present invention can capture and release cells or vesicles in a solution, and further process and analyze the obtained cells or vesicles.

Provided are: a production device by which a cell structure having a three-dimensional structure is produced using a plurality of linear members; a production system therefor; and a production method therefor. The production device 100 comprises a top plate 110, pins 120A to 120D, a first slide plate 130, a second slide plate 140, a stopper 150, a base plate 160, an outer peripheral needle-shaped member 170 and an inner peripheral needle-shaped member 180. Cell aggregates 400 are put into a three-dimensional tubular space S1 that is defined by the outer peripheral needle-shaped member 170 and the inner peripheral needle-shaped member 180. Then, the top plate 110 is pressed downward on the accumulated cell aggregates 400. Thus, the cell aggregates 400 are immersed in a culture solution 210 and stuck together so that a tubular cell structure 500 is produced using the three-dimensional space S1 as a mold.

A system for introducing a vector into includes a filter module defining an intra-capillary space and an extra-capillary space separated from the intra-capillary space by a porous membrane. The system also includes a pair of intra-capillary ports fluidly coupled to opposite ends of the intra-capillary space and each receiving a transduction media, cells, and a vector. The system also includes a pair of extra-capillary ports coupled to opposite ends of the extra-capillary space and in fluid-communication with a source of extra-capillary media and a waste container.

The present invention relates to a novel bioreactor system for preparing an engineered three- dimensional biological tissue construct. The bioreactor system comprises a cultivation chamber that is designed to allow the formation, cultivation and the subsequent testing and/or stimulation of tissue constructs on one or more support elements with a minimum risk of microbial contamination or mechanical damage The invention furthermore relates to a method for preparing an engineered tissue construct using the novel bioreactor system. The invention also relates to the use of the bioreactor system for preparing engineered biological tissue constructs, preferably tissue constructs which are suitable for being used in clinical tissue replacement and reconstructive therapy, drug development, drug screening, toxicity testing, cosmetic studies, safety testing, developmental studies, disease modeling, or food purposes.

A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

Disclosed herein are compositions, devices, methods, processes, and systems for culturing of large quantities of mammalian cells in suspension culture. Also disclosed are unique and surprising cell populations derived from the disclosed methods, processes, and systems. In many embodiments, the cells are cultured in suspension in liquid culture media that is agitated to maintain the cells in suspension, and agitation increases to maintain cells in suspension while growing and dividing to create cell spheres. The disclosed devices, methods, processes, and systems are useful in tailoring characteristics of the resulting cells based on characteristics of donor subject/initial cells. The disclosed cell populations are useful in treating subjects with cell based therapies in need thereof for various diseases and conditions.