A bioreactor cleaning system for cleaning a bioreactor in a rail vehicle. A suction unit, a supply unit, an electronic control unit for actuating the suction unit and the supply unit, an acid tank, a collection tank for receiving a liquid suctioned out of the bioreactor, and a freshwater connection are provided. The bioreactor cleaning system comprises a metering unit which has acid canister connections, base canister connections, an acid metering device which can be connected to the acid canister connections and the acid tank, and a base metering device which can be connected to the base canister connections and the acid tank. Additionally, a mixer is provided in order to mix the different liquids.

A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.

A single use probe, sterilizable by irradiation, for a single use component for use in a biopharmaceutical process, comprises at least one sensor relevant for the biopharmaceutical process, an RFID tag and a memory rewritable in principle, in which data with respect to an integrity check of the single use probe are stored. A method for quality assurance of such a single use probe comprises: providing the probe with an RFID tag and a memory rewritable in principle, in particular a FeRAM memory as part of the RFID tag; defining a measurement-principle-specific quality parameter of the single use probe; defining a tolerance value for the parameter; performing an integrity check of the probe by first determining and writing into the memory values of the defined quality parameter before sterilization of the probe by irradiation; determining the values of the defined quality parameter after irradiation; and comparing the values of the quality parameter determined before radiation to those determined after.

Herein is reported a bioreactor comprising a cultivation vessel and a reactor head plate, wherein the cultivation vessel has a working volume of from 20 ml to 350 ml and comprises two or more in-situ sensors, wherein the reactor head plate comprises an in-situ sensor port, wherein to the in-situ sensor port at least one in-situ glucose sensor and one in-situ pH sensor are connected.

The invention relates to a method for the biological in-situ methanation of CO.sub.2 and H.sub.2 in a bioreactor. The method includes feeding an organic substrate into the bioreactor wherein at least part of the organic substrate is converted to a biogas comprising methane and carbon dioxide by means of microorganisms. The organic substrate includes crude fiber and at least 0.15 kg of crude fiber per m.sup.3 bioreactor volume per day is fed into to the bioreactor. The bioreactor is operated at between about 20-45° C. H.sub.2 is fed to the CO.sub.2 into the bioreactor to produce methane.

A method and a device allow measurement of the pH value of a solution. The method includes illuminating a solution containing a pH indicator and a pH changer with light emitted from a light emitting element, receiving light transmitted through the solution with a light receiving element, measuring absorbance with monochromatic light selectively from the received transmitted light, and calculating a pH value corresponding to the measured absorbance based on a predefined absorbance table. The device includes an illuminator that illuminates a solution containing a pH indicator and a pH changer with light emitted from a light emitting element, a light receiver that receives light transmitted through the solution, a measurer that measures absorbance with light with a wavelength of monochromatic light selectively from the received transmitted light, and a calculator that calculates a pH value corresponding to the absorbance measured by the measurer based on a predefined absorbance table.

The present set of embodiments relate to a system, method, and apparatus controlling a cell culture media mixing system. The control system includes an integrated control unit capable of controlling a broad ranges of peripheral devices commonly used in bioproduction through a graphical user interface displayed on a touch sensitive screen. The bioproduction system is designed to be highly customizable through process modification and recipe creation by user with little or no knowledge of programming and is capable of controlling a wide variety of devices using a single unit. The bioproduction system allows for auto-detection, auto-calibration, and automatic of device related processes into a bioproduction workflow.

The invention relates to the production of microalgae biomass. The microalgae contained in a suspension of water and microalgae are continuously phototrophically or mixotrophically cultivated in a cultivation module (1), which is passed multiple times by the suspension and has a gas part and a liquid part with a liquid supply (3), by supplying light from at least one artificial light source (5) and nutrients. According to the turbidity established by sensors, volume fractions of the suspension are repeatedly discharged from the cultivation module (1) for the harvest of microalgae and removed by means of a centrifuge (7). The cultivation of the microalgae occurs in an climate chamber forming the cultivation module (1), which is operated using water. Alongside a regulating of the temperature of the suspension, there also occurs a regulating of its pH value via the controlled addition of buffer ions and a regulating of the redox potential of the suspension and thereby also of its microbial contamination by controlling the light and nutrient supply, as well of a metered addition of oxygen. In addition, after the removal of microalgae, the remaining suspension is irradiated with UV light in order to kill unwanted microbial contamination before being returned into the cultivation module (1).

The present invention relates to methods and systems for performing perfusion cell culture whereby the supernatant of the bleed stream is recovered.

A control device performs control of a culture condition in production of an organic compound by a fermentation method. The control device executes processing a plurality of times to acquire culture data, to calculate, using the acquired culture data, a plurality of candidates for a culture condition set in advance, and a linear model set in advance that outputs a production amount of an organic compound at a future time, the production amount at the future time for each of the candidates, to determine an optimum candidate out of the candidates using the calculated production amount at the future time for each of the candidates and a target production amount of the organic compound at the future time set in advance, and to change the culture condition to the determined candidate. The linear model and the target production amount are set for each time.