The invention relates to the field of lyophilizates of biological materials. More particularly, the invention relates to a new method for preparing a lyophilizate of cells comprising a step of cryogenics “under pressure”. In a preferred embodiment, this method is applied to lactic acid bacteria.

The invention relates to the field of lyophilizates of biological materials. More particularly, the invention relates to a new method for preparing a lyophilizate of cells comprising a step of cryogenics “under pressure”. In a preferred embodiment, this method is applied to lactic acid bacteria.

Systems and methods for predicting a patient response to various agents and/or combinations of agents using ex vivo dosing and imaging are disclosed. In one example, a method of determining treatment efficacy includes analyzing a solid cell culture over time, e.g., first and second responses to a solid cell culture to respective treatments may be compared to determine a treatment efficacy of each treatment. Systems and methods for applying the treatments to the cell culture and analyzing the cell culture and efficacy are disclosed.

Compositions are provided that contain sporulated coccidial oocysts, wherein the compositions are free of antibiotics and comprise formalin at a concentration sufficient to inhibit microbial growth for at least 12 months while maintaining oocyst viability. Methods of preparing such compositions are also provided.

Compositions are provided that contain sporulated coccidial oocysts, wherein the compositions are free of antibiotics and comprise formalin at a concentration sufficient to inhibit microbial growth for at least 12 months while maintaining oocyst viability. Methods of preparing such compositions are also provided.

The present invention provides a solution for cryopreservation of animal cells or animal tissues which substantially includes a cryoprotectant that contains 0.5 w/v % to 6.0 w/v % of dextran or derivatives thereof or salts thereof and contains dimethyl sulfoxide, and includes a physiological aqueous solution; a cryopreserved product of animal cells or animal tissues which includes animal cells or animal tissues, and includes the above-described solution for cryopreservation of animal cells or animal tissues; and a cryopreservation method of animal cells or animal tissues, which is a method using the above-described solution for cryopreservation of animal cells or animal tissues.

The present invention provides a solution for cryopreservation of animal cells or animal tissues which substantially includes a cryoprotectant that contains 0.5 w/v % to 6.0 w/v % of dextran or derivatives thereof or salts thereof and contains dimethyl sulfoxide, and includes a physiological aqueous solution; a cryopreserved product of animal cells or animal tissues which includes animal cells or animal tissues, and includes the above-described solution for cryopreservation of animal cells or animal tissues; and a cryopreservation method of animal cells or animal tissues, which is a method using the above-described solution for cryopreservation of animal cells or animal tissues.

The present invention relates to a method of storing a biocatalyst, which biocatalyst is capable of converting acrylonitrile to acrylamide, comprising the steps: (a) providing an aqueous suspension comprising the biocatalyst, which is capable of converting acrylonitrile to acrylamide, which aqueous suspension is an aqueous fermentation broth; (b) sequentially in either order or simultaneously (b) concentrating the aqueous suspension comprising the biocatalyst to a concentration of at least 3% (w/w); and (b2) reducing the temperature of the aqueous suspension comprising the biocatalyst to a temperature of below 8° C., thereby forming a concentrated aqueous suspension; and (c) maintaining the concentrated aqueous suspension of step (b) at a temperature of below 8° C. The invention also relates to a biocatalyst composition obtainable by this method and also the use of the biocatalyst composition in a process of preparing (meth-) acrylamide aqueous solution from (meth-) acrylonitrile. The invention further relates to a method for producing an aqueous (meth-) acrylamide solution comprising the aforementioned storage method and also to a method for producing polyacrylamide comprising the aforementioned storage method.

The present invention relates to a method of storing a biocatalyst, which biocatalyst is capable of converting acrylonitrile to acrylamide, comprising the steps: (a) providing an aqueous suspension comprising the biocatalyst, which is capable of converting acrylonitrile to acrylamide, which aqueous suspension is an aqueous fermentation broth; (b) sequentially in either order or simultaneously (b) concentrating the aqueous suspension comprising the biocatalyst to a concentration of at least 3% (w/w); and (b2) reducing the temperature of the aqueous suspension comprising the biocatalyst to a temperature of below 8° C., thereby forming a concentrated aqueous suspension; and (c) maintaining the concentrated aqueous suspension of step (b) at a temperature of below 8° C. The invention also relates to a biocatalyst composition obtainable by this method and also the use of the biocatalyst composition in a process of preparing (meth-) acrylamide aqueous solution from (meth-) acrylonitrile. The invention further relates to a method for producing an aqueous (meth-) acrylamide solution comprising the aforementioned storage method and also to a method for producing polyacrylamide comprising the aforementioned storage method.

The present invention provides novel Lactococcus lactis lactic acid bacterium strains having improved texturing properties and methods of using the strains for producing food products.