A non-destructive and reversible process to deodorize Nannochloropsis algae is provided.

The invention discloses the use of the Schizochytrium limacinum and its preparation in improving the quality and yield of animal product. The deposit number of Schizochytrium limacinum in the present invention is CGMCC No. 13746 in the China General Microbiological Culture Collection Center. The Schizochytrium limacinum powder produced by the Schizochytrium limacinum may increase the DHA content in an animal product, reduce the cholesterol content in an animal product, and also improve the egg production performance of poultry. This animal product with high DHA content from natural sources is organic, safe, stable, and easy to be absorbed. It may be used as a safer and effective way for people to ingest natural DHA, and it may also cater to and meet consumer needs. Thus, Schizochytrium limacinum and Schizochytrium limacinum powder of the present application have a wide range of application in the field of general food and livestock breeding.

Provided is a method for producing astaxanthin, comprising: (a) performing heterotrophic cultivation of astaxanthin-producing Haematococcus pluvialis; (b) performing heterotrophic cultivation of the Haematococcus pluvialis obtained in step (a) under conditions of high acetate concentration and high dissolved oxygen concentration, where the acetate concentration is at least 1800 mg/L and the dissolved oxygen concentration is at least 2.0 mg/L; and (c) collecting algae cells from step (b) and/or harvesting astaxanthin. Also provided is a base medium for culturing Haematococcus pluvialis, a propagation feed medium, and an induction feed medium.

A method for producing astaxanthin, comprising: (a) acquiring vegetative cells of astaxanthin-producing Haematococcus pluvialis; (b) heterotrophically culturing the vegetative cells of astaxanthin-producing Haematococcus pluvialis in a nutrient-poor culture medium containing an organic carbon source and under a no-light condition, to obtain spore cells; and (c) harvesting the spore cells and/or astaxanthin, and optionally purifying the astaxanthin. Also provided is a culture medium used in the described method.

Water-responsive actuators and methods for creating water responsive actuators are disclosed. In some embodiments, the disclosed subject matter includes a first layer, for example a plastic tape, and a second layer, for example bacterial spores and cured adhesive. The second layer can be created in a pattern. The pattern can include joints, which can contract when exposed to dry air and can thereby bend the actuator, and can expand when exposed to humid air and thereby return the actuator its original position.

The present disclosure provides a composition comprising: an extracellular polymeric substance produced by microalgae; plant growth promoting Gram-negative bacteria; and an agriculturally acceptable carrier. Also provided are an isolated biologically pure culture of Parachlorella kessleri Accession No. NCMA 202103001, a mutant thereof having all the identifying characteristics thereof, or a cell-free preparation or extracellular polymeric substance thereof, which may be used for plant enhancement and improving health of soil.

The invention is directed to Haematococcus sp. KAU-01 as well as to a culture medium for Haematococcus sp. KAU-01, and to methods for using this strain to process environmental pollutants such as gases generated by coal-fired plants.

The present invention is directed to isolated microorganisms as well as strains and mutants thereof, biomasses, microbial oils, compositions, and cultures; methods of producing the microbial oils, biomasses, and mutants; and methods of using the isolated microorganisms, biomasses, and microbial oils.

A method for producing biomass from a microalgae includes culturing the microalgae in an effluent diluted in seawater. A method for bioremediating an effluent includes culturing a microalgae in the effluent diluted in seawater. The microalgae is at least one of a strain of the genus Nodularia, a strain of the genus Chrysoreinhardia, a strain of the genus Halochlorella, or combinations thereof. At the beginning of culturing, the diluted effluent exhibits concentrations of total nitrogen (N) in the range of 30-150 mg/l and concentrations of total phosphorus (P) in the range of 1-15 mg/l. The N/P quotient is in the range of 5-40.

Provided is a component having plant defense activity. A PR1 gene expression activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant defense activator containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; A plant disease preventive or ameliorating agent containing a culture of a unicellular alga containing a green photosynthetic pigment as an active ingredient; and a method of producing a plant defense activating component containing: cultivating a unicellular alga containing a green photosynthetic pigment under an aerobic condition to produce a culture containing an extracellular polysaccharide.