The present invention is concerned with the provision of means and methods for gene expression. Specifically, it relates to a polynucleotide comprising an expression control sequence which allows for seed specific of a nucleic acid of interest being operatively linked thereto in plants. Furthermore, vectors, host cells, transgenic plants and methods for expressing nucleic acids of interest are provided which are based on the said polynucleotide.

The present invention is directed to a transgenic soybean plant having increased seed protein content and/or increased seed oil content comprising a polynucleotide encoding a β-ConGlycinin soybean seed storage promoter that functions in the soybean plant operably linked to a polynucleotide encoding a soybean seed storage polypeptide having β-ConGlycinin activity. The invention is further directed to a method of increasing seed protein content and/or increasing seed oil content of a soybean plant comprising transforming the soybean plant with a polynucleotide encoding a β-ConGlycinin soybean seed storage promoter that functions in the soybean plant operably linked to a polynucleotide encoding a soybean seed storage polypeptide having β-ConGlycinin activity.

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

A method of modifying the amount of at least one biochemical component in a plant comprising expressing Qua-Quine Starch (QQS) in the plant, the wild-type of which does not express QQS; a transgenic plant, or part thereof, which comprises and expresses QQS as a transgene and in which the amount of at least one biochemical component is modified; a tissue culture of regenerable cells of the transgenic plant; a vector comprising a nucleotide sequence, which encodes the coding sequence of QQS, operably linked to a non-native promoter, which promotes expression of the nucleotide sequence in a plant, which is other than Arabidopsis; and a method of producing a food or industrial product from a plant.

Provided are methods and/or systems having advantages of cost effective, time saving, and informative user-friendly characteristics to accomplish trait introgression. The methods provided comprise determining presence of omega-3 fatty acids (for example docosahexanoic acid or DHA; docosapentaenoic acid or DPA; Alpha linolenic acid or ALA; and eicosapentaenoic acid or EPA) using Fourier Transformed Infra Red (FTIR) spectrum. The use of FTIR enables analysis of the oil contained in the seeds using a multivariate-based Mid-FTIR model. The methods and/or systems provided advantages of non-destructive analysis to provide information to facilitate trait introgression and other breeding applications.

An isolated protein that is highly abundant in the seed of wheat varieties with desirable breadmaking qualities is provided. Associated promoters and promoter active fragments are also provided. Further provided are plants or plant parts with improved or enhanced breadmaking properties, and methods of producing such plants, wherein the abundance of the isolated protein is increased or enhanced. Such plants and/or plant parts may be used in the production of plant products, for example bread and read products.

The present invention relates to methods for the design and production of synthetic promoters with a defined specificity and promoters produced with these methods.

The present invention is in the field of plant molecular biology and provides methods for production of high expressing constitutive promoters and the production of plants with enhanced constitutive expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to said promoters and/or introduced into plants.

The present invention pertains to a method of expressing a protein of interest, preferably a heterologous protein, in preferably a plant. In a preferred embodiment said plant is a doubled haploid homozygous transgenic Nicotiana tabacum plant silenced for Ntp303. Furthermore, the invention relates to said plant with or without nucleic acid constructs according to the invention. Propagation, harvest and tissue material of said transgenic Nicotiana tabacum plant is also a part of the invention.

The invention relates to plants with increased resistance to plant pathogens, wherein the intracellular concentration of inositol pyrophosphate InsP.sub.7 and/or InsP.sub.8 in said plants is increased in comparison to the wild-type plant. In particular, the invention involves plants with increased expression of at least one protein involved in the synthesis of inositol pyrophosphates InsP.sub.7 and/or InsP.sub.8, such as, for example, proteins VIH2 and VIH1. The plants according to the invention are particularly resistant to the following plant pathogens: herbivore insects, for example larvae of agriculturally relevant pests, pathogenic fungi, such as necrotrophic fungi, or other plant pests, such as biotrophic pathogens. The invention further relates to the method for increasing plant resistance to plant pathogens, wherein the intracellular concentration of inositol pyrophosphates InsP.sub.7 and/or InsP.sub.8 is increased in comparison to the wild-type plant.