Several embodiments relate to methods of repairing and/or regenerating damaged or diseased tissue comprising administering to the damaged or diseased tissues compositions comprising exosomes. In several embodiments, the exosomes comprise one or more microRNA that result in alterations in gene or protein expression, which in tum result in improved cell or tissue viability and/or function.

The invention discloses a device and method for producing and purifying exosomes from progenitor cells using a closed and sterile circuit having a pumping means and a plurality of serially connected processing stations.

The purpose of the present invention is to provide a cardiac cell culture material which specifically acts on cardiac cells. In addition, another purpose of the present invention is to provide artificial organ material obtained by culturing by using said cardiac cell culture material, and a method for producing the same. Thus, provided is a cardiac cell culture, wherein functional cardiac tissue is favorably built by using a cardiac cell culture material containing VCAM-1.

Various embodiments are described herein for creating a 3D human heart model for modelling arrythmias, wherein the method comprises seeding a structure with a mixture of human cardiomyocytes, cardiac fibroblasts and a fibrin mixture to form cardiac tissue; applying a plating media for settlement and compaction of the cardiac tissue; and adding an arrhythmogenic media to the cardiac tissue, where the arrhythmogenic media comprises methyl-beta cyclodextrin for disrupting calcium signaling.

Methods fabricate an endothelialized myocardium usable for screening of a drug. A microfibrous hydrogel scaffold is manufactured with additive manufacturing that concurrently bioprints endothelial cells directly within the microfibrous hydrogel scaffold. A bioink is bioprinted into an arrangement of one or more microfibers. The bioink includes at least one crosslinking component and suspended endothelial cells. The crosslinking component or components are crosslinked to yield the microfibrous hydrogel scaffold having the endothelial cells embedded directly within. The microfibrous hydrogel scaffold is seeded with cardiomyocytes to yield the endothelialized myocardium with a controlled anisotropy. The endothelialized myocardium can be incubated until the endothelialized myocardium matures into spontaneously beating myocardial tissue having contractions aligned with the controlled anisotropy. The beating myocardial tissue can be used to screen a drug when the beating myocardial tissue is embedded within a microfluidic perfusion bioreactor.

Cardiac neural crest cells and methods for making and using the same to effect cardiac injury is described herein.

Provided are methods and materials for assaying known and candidate therapeutics for inotropic cardiac effects in one or more in vitro assay formats comprising preloaded cardiac tissues and/or organoids.

Steam cell and exosome compositions via combination therapy, related gene therapy and pluripotent stem cell derived muscle regeneration as having therapeutic utility to treat a variety of diseases and disorders, e.g., cardiovascular disease, Duchenne muscular dystrophy, and fibrotic disease.

A diode and logic gate comprising cells is disclosed. A method of making the diode and logic gate comprising cells is disclosed.

The disclosure provides a device for developing organized tissue strips, such as cardiac tissue strips, for high-throughput assays of functional performance and the methods involved in fabricating, assembling, implementing, utilizing and analyzing data from such assays. The disclosure further provides systems for constructing such devices, systems comprising those devices comprising cells and extracellular matrix material for developing organized tissue strips or comprising the devices and organized tissue strips. The disclosure further provides methods for assaying a property of a tissue strip, such as contractile force.