Process for the preparation of a galacto-oligosaccharide preparation, which process comprises the step of contacting a lactulose-containing feed with a beta-galactosidase derived from Papiliotrema terrestris. The resulting galacto-oligosaccharide is acceptable to subjects suffering from GOS-related allergy and subjects having lactose intolerance.

An enzymatic process for the preparation of C16 alkyl polyglycosides and/or C18 alkyl polyglycosides by reacting C16 alkyl glycoside and/or C18 alkyl glycoside with a glycosyl donor containing monosaccharide residues to form an alkyl polyglycoside intermediate which can be fractionated to form an alkyl polyglycoside product, wherein the mole-average degree of polymerization mean DP) of the glycoside chains is greater than or equal to 3.0 units and the molar concentration of alkyl triglycoside (DP3) is greater than alkyl monoglycoside (DPI). The C16/C18 alkyl polyglycoside product is particularly useful in health care formulations, especially in combination with and/or as a solubilizer for active pharmaceutical ingredients (APIs).

A process for the preparation of C4 to C24 alkyl polyglycosides by the use of an enzyme to react C4 to C24 alkyl glycoside with a glycosyl donor containing monosaccharide residues, wherein the C4 to C24 alkyl polyglycosides have a mole-average degree of polymerization (mean DP) of the glycoside chains of greater than 1.5 units. The C4 to C24 alkyl polyglycosides are particularly useful in personal care formulations.

A process for producing a solid material comprising isomaltulose crystals and trehalulose, including: A) contacting an enzyme complex with a sucrose-containing solution; B) isomerizing at least a part of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to obatin a solution including isomaltulose, trehalulose and water; D) partially removing the water by evaporation to obtain a concentrated solution; E) bringing the concentrated solution to a temperature range of 30° C. to 63° C. and subsequently inducing isomaltulose crystallization in this temperature range followed by cooling, thereby obtaining a solid material including isomaltulose crystals and trehalulose.

A process for producing a solid material comprising isomaltulose crystals and trehalulose, including: A) contacting an enzyme complex with a sucrose-containing solution; B) isomerizing at least a part of the sucrose to isomaltulose and trehalulose; C) separating off the enzyme complex to obatin a solution including isomaltulose, trehalulose and water; D) partially removing the water by evaporation to obtain a concentrated solution; E) bringing the concentrated solution to a temperature range of 30° C. to 63° C. and subsequently inducing isomaltulose crystallization in this temperature range followed by cooling, thereby obtaining a solid material including isomaltulose crystals and trehalulose.

The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered host cells. The present invention describes a method of making sialylated oligosaccharide by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for an enzyme involved in sialylated oligosaccharide synthesis and at least one nucleic acid expressing a membrane protein.

The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered host cells. The present invention describes a method of making sialylated oligosaccharide by fermentation with a genetically modified cell, as well as to the genetically modified cell used in the method. The genetically modified cell comprises at least one nucleic acid sequence coding for an enzyme involved in sialylated oligosaccharide synthesis and at least one nucleic acid expressing a membrane protein.

A technique for processing ancient, heritage and modern wheat, grains, seeds, beans, legumes, tuber and root vegetables create baking flours suitable for human consumption. The initial ingredient is incubated to initiate germination and activate internal enzymes and nutrient production for useful enzymes, proteins and nutrients. Germination is terminated and the product wet-milled to fracture or shear the outer hull, exposing the inner grain. The product is mixed with water at varying temperatures during which amylase is added. The mixture is incubated to facilitate saccharification of starches into sugars by the amylase enzymes. The mixture is pasteurized to denature the amylases and the mash pressed and/or strained to separate the liquid and solids. The solid phase is dried and milled into higher fiber, high protein, low carbohydrate flour. The liquid is carbohydrate-rich with substantial fiber, protein and other nutrients dissolved in the solution.

A technique for processing ancient, heritage and modern wheat, grains, seeds, beans, legumes, tuber and root vegetables create baking flours suitable for human consumption. The initial ingredient is incubated to initiate germination and activate internal enzymes and nutrient production for useful enzymes, proteins and nutrients. Germination is terminated and the product wet-milled to fracture or shear the outer hull, exposing the inner grain. The product is mixed with water at varying temperatures during which amylase is added. The mixture is incubated to facilitate saccharification of starches into sugars by the amylase enzymes. The mixture is pasteurized to denature the amylases and the mash pressed and/or strained to separate the liquid and solids. The solid phase is dried and milled into higher fiber, high protein, low carbohydrate flour. The liquid is carbohydrate-rich with substantial fiber, protein and other nutrients dissolved in the solution.

This disclosure is in the technical field of synthetic biology and metabolic engineering. The disclosure provides engineered viable bacteria having a reduced or abolished synthesis of poly-N-acetyl-glucosamine (PNAG), Enterobacterial Common Antigen (ECA), cellulose, colanic acid, core oligosaccharides, Osmoregulated Periplasmic Glucans and Glucosylglycerol (O), glycan, and trebalose. The disclosure further provides methods for the production of bioproduct by the viable bacteria and uses thereof. Furthermore, the disclosure is in the technical field of fermentation of metabolically engineered microorganisms producing bioproduct.