Disclosed is a technique for constructing optogenetic amplifier and inverter circuits utilizing transcriptional activator/repressor pairs, in which expression of the transcriptional activator or repressor, respectively, is controlled by light-controlled transcription factors. This system is demonstrated utilizing the quinic acid regulon system from Neurospora crassa, or Q System, a transcriptional activator/repressor system. This is also demonstrated utilizing the galactose regulon from Saccharomyces cerevisiae, or GAL System. Such optogenetic amplifier circuits enable multi-phase microbial fermentations, in which different light schedules are applied in each phase to dynamically control different metabolic pathways for the production of proteins, fuels or chemicals. The orthogonal nature of the Q and GAL systems enable the co-expression of amplifier and inverter circuits to simultaneously amplify and invert the response of light-controlled transcriptional controls over different sets of genes in the same cell.

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

Yeast cells are genetically modified to disrupt a native metabolic pathway from dihydroxyacetone to glycerol. In certain aspects, the yeast cell is of the genera Kluyveromyces, Candida or Issatchenkia. In other aspects, the yeast cell is capable of producing at least one organic acid, such as lactate. The yeast cells produce significantly less glycerol than the wild-type strains, and usually produce greater yields of desired fermentation products. Yeast cells of the invention often grow well when cultivated, despite their curtailed glycerol production.

The present invention concerns the field of processed cheese compositions, and, more particularly, relates to reduced salt processed cheese compositions. The present invention derives from the surprising finding that potassium lactate can be used in the manufacture of processed cheese compositions with reduced sodium content, without detrimental consequences on the taste of the final product. A processed cheese composition according to the invention imparts to the final product an improved flavour perception, as well as strengthening salty, savoury and cheesy taste. An additional surprising finding of the method of the invention is that the use of potassium lactate according to the invention has beneficial consequences on the safety and shelf life of the final product. The use of potassium lactate, in particular potassium lactate in the form of a fermentation product in accordance with the invention, also obviates the need to incorporate artificial flavorings and flavor enhancers, which results in a product with an improved label.

The present invention concerns the field of processed cheese compositions, and, more particularly, relates to reduced salt processed cheese compositions. The present invention derives from the surprising finding that potassium lactate can be used in the manufacture of processed cheese compositions with reduced sodium content, without detrimental consequences on the taste of the final product. A processed cheese composition according to the invention imparts to the final product an improved flavour perception, as well as strengthening salty, savoury and cheesy taste. An additional surprising finding of the method of the invention is that the use of potassium lactate according to the invention has beneficial consequences on the safety and shelf life of the final product. The use of potassium lactate, in particular potassium lactate in the form of a fermentation product in accordance with the invention, also obviates the need to incorporate artificial flavorings and flavor enhancers, which results in a product with an improved label.

The present invention discloses a genetically engineered Kluyveromyces sp. yeast cell comprising at least a genetic modification that inactivates or deletes a nucleic acid sequence encoding for transcription factor SEQ ID No. 2, particularly Haal. The genetically engineered yeast cell according to this invention has an improvement of lactic acid tolerance, lactic acid production or a combination thereof as compared to the parental.

The present invention relates to a method of extracting a low-molecular-weight proanthocyanidin using cranberries and lactic acid bacteria, and more particularly, the method may include (S1) growing and extracting a seed culture from cranberries, (S2) reinforcing the metabolism of a saccharide by adjusting a metabolic process, (S3) extracting the seed culture whose metabolic process is adjusted and culturing the extracted result, (S4) mixing cranberries, distilled water and a saccharide with the grown seed culture and fermenting the mixture, and (S5) extracting an unbound polyphenol from the fermented mixture.

The present invention relates generally to the field of industrial biotechnology and particularly to an improved hydrolysis method for increasing sugar production from a high solids concentration of lignocellulosic biomass, especially one derived from Municipal Solid Waste (MSW) by enzymatic hydrolysis of a lignocellulosic biomass to obtain a slurry, wherein the hydrolysis comprises aliquot additions of enzyme and lignocellulosic biomass; and removal of sugars from the slurry and washing of the residual lignocellulosic biomass.

The present invention relates generally to the field of industrial biotechnology and particularly to an improved hydrolysis method for increasing sugar production from a high solids concentration of lignocellulosic biomass, especially one derived from Municipal Solid Waste (MSW) by enzymatic hydrolysis of a lignocellulosic biomass to obtain a slurry, wherein the hydrolysis comprises aliquot additions of enzyme and lignocellulosic biomass; and removal of sugars from the slurry and washing of the residual lignocellulosic biomass.

Provided herein are compositions and methods for production of organic acids. In particular, provided herein are consolidated bioprocessing compositions and methods for single reactor production of organic acids.