A sensor implanted in tissues and including a sensing layer is fabricated by mixing the signal transduction enzyme with non-reactive components including buffer salts and fillers, and spin coating the enzyme onto a substrate. The signal transduction enzyme is crosslinked by introducing the coated substrate in a vacuum chamber. In the chamber, a crosslinker evaporates and is deposited onto the enzyme, therefore crosslinking the enzyme.

Disclosed are devices, systems and methods for in vivo monitoring of localized environment conditions within a patient user by measuring analytes, including glucose, oxygen, and/or other analytes. In some aspects, a sensor device includes a wafer-based substrate, at least one electrochemical sensor two-electrode contingent including a working electrode and a reference electrode on the substrate and configured to detect a target analyte in a body fluid when the sensor device is deployed within a subject's body, where the working electrode is functionalized by a chemical layer configured to facilitate a reaction involving the target analyte that produces an electrical signal; and an electronics unit in communication with the electrochemical sensor electrode contingent to transmit the electrical signal to an external processor.

Nanozymes capped with a radical-scavenging capping agent are disclosed for use in biosensing assays with improved sensitivity. The radical-scavenging capping agent facilitates the capture and retention of one or more radicals for enhancing a catalytic reaction. In some example embodiments, the nanozyme capped by the radical-scavenging capping agent is capable of catalyzing the decomposition of hydrogen peroxide or molecular oxygen. The capped nanozymes may be incorporated with an electrode, such as the working electrode of an electrochemical sensor, for achieving enhanced catalytic activity and a lower limit of detection. In some example embodiments, the radical-scavenging capping agent is or includes thiocyanate. A rapid ethanol detection device and associated method are described in which the working electrode of an electrochemical sensor is modified by a peroxidase-mimetic nanozyme capped with a radical-scavenging capping agent for the enhanced generation of a reduction current associated with the decomposition of hydrogen peroxide.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P).sup.+) or derivative thereof; an NAD(P).sup.+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

A biosensor for the rapid, inexpensive, quantitative, and convenient detection of SARS-CoV-2 biomarkers, methods of manufacturing, and methods of using the same, to identify a patient's prognosis and past/present SARS-CoV-2 infection status, wherein the biosensor comprises a microfluidics layer, a multimodal sensing layer comprising two or more working electrodes, and a logic circuit that may include a processor and non-transitory memory with computer executable instructions embedded thereon.

A method for quantifying a target substance, comprising: bringing a sample containing the target substance into contact with a biosensor which comprises an enzyme electrode containing an oxidoreductase and a counter electrode; measuring a change in the potential difference between the enzyme electrode and the counter electrode due to oxidation reaction of the target substance catalyzed by the oxidoreductase; and calculating the concentration of the target substance based on the change in the potential difference; wherein a potential is applied between the enzyme electrode and the counter electrode before the measurement of the change in the potential difference.

NADP-dependent oxidoreductase compositions, and electrodes, sensors and systems that include the same. Analyte sensors include an electrode having a sensing layer disposed thereon, the sensing layer comprising a polymer and an enzyme composition distributed therein. The enzyme composition includes nicotinamide adenine dinucleotide phosphate (NAD(P).sup.+) or derivative thereof; an NAD(P).sup.+-dependent dehydrogenase; an NAD(P)H oxidoreductase; and an electron transfer agent comprising a transition metal complex.

The present disclosure relates to metal alloys for biosensors. An electrode is made from ruthenium metal or a ruthenium-based alloy. The resulting electrode has physical and electrical property advantages when compared with existing pure metal electrodes.

The present invention relates: to a novel transition metal complex having a C—N ligand, which can be used for various devices including an electrochemical sensor, to a device comprising same; and preferably, to an electrochemical sensor.

An electrode measuring the presence of an analyte is described as one embodiment. The electrode includes a working conductor with an electrode reactive surface and a first reactive chemistry that is responsive to the analyte. The electrode further includes a first transport material that enables flux of the first analyte to the first reactive chemistry and a second transport material that supplies a reactant to the first reactive chemistry. Wherein the first reactive chemistry does not contact the electrode reactive surface while at least partially shadowing a portion of the electrode reactive surface.