The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P.sub.1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P.sub.1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

The present specification discloses SNAP-25 compositions, methods of making α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P.sub.1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, α-SNAP-25 antibodies that bind an epitope comprising a carboxyl-terminus at the P.sub.1 residue from the BoNT/A cleavage site scissile bond from a SNAP-25 cleavage product, methods of detecting BoNT/A activity, and methods of detecting neutralizing α-BoNT/A antibodies.

New use of ADAMTS13 in the clinical filed is provided. The use of ADAMTS13 as a biomarker for monitoring the onset of liver damage, hepatic ischemia/reperfusion injury or the liver function after liver transplantation: a method of testing liver damage, a method of testing hepatic ischemia/reperfusion injury, or a method of testing the liver function after liver transplantation, each of the methods comprising measuring or monitoring the ADAMTS13 activity in a sample from a mammal; an agent for treating diseases selected from the group consisting of liver damage, hepatic ischemia/reperfusion injury and hepatic dysfunction after liver transplantation, which comprises ADAMTS13 or a mutant of ADAMTS13 as an effective ingredient.

A microwave microstrip resonator apparatus including a housing; a resonator within the housing; an output conductor within the housing and spaced apart from the resonator so as to define a capacitive gap therebetween; a reaction vessel configured to reside with the capacitive gap; and a power supply coupled to the resonator whereby contents within the reaction vessel are heated when energy is supplied to the resonator by the power supply. A mass spectrometer may also be coupled to an outlet end of the reaction vessel such that the contents within the reaction vessel are, simultaneously, delivered to the mass spectrometer for analysis.

A microwave microstrip resonator apparatus including a housing; a resonator within the housing; an output conductor within the housing and spaced apart from the resonator so as to define a capacitive gap therebetween; a reaction vessel configured to reside with the capacitive gap; and a power supply coupled to the resonator whereby contents within the reaction vessel are heated when energy is supplied to the resonator by the power supply. A mass spectrometer may also be coupled to an outlet end of the reaction vessel such that the contents within the reaction vessel are, simultaneously, delivered to the mass spectrometer for analysis.

The present invention relates to protein sequencing, more particularly the invention discloses improved aminopeptidases for single molecule protein sequencing and/or amino acid identification. Said aminopeptidases can enzymatically cleave off N-terminal amino acids and are highly suitable in a kinetics-based peptide sequencing approach. Based on the kinetics of the cleaving reaction or of the engagement between said aminopeptidases and peptide to be sequenced, information on the identity of the cleaved amino acids is provided.

The present invention relates to protein sequencing, more particularly the invention discloses improved aminopeptidases for single molecule protein sequencing and/or amino acid identification. Said aminopeptidases can enzymatically cleave off N-terminal amino acids and are highly suitable in a kinetics-based peptide sequencing approach. Based on the kinetics of the cleaving reaction or of the engagement between said aminopeptidases and peptide to be sequenced, information on the identity of the cleaved amino acids is provided.

Methods for analysis of a glucuronide ligand-drug conjugate are provided.

Methods for analysis of a glucuronide ligand-drug conjugate are provided.

An object of the present invention is to provide a standard substance for quantification of PSA having a specific sugar chain that can be used in a general purpose quantification, wherein the standard substance has less unbalanced sugar chain expression patterns, can be manufactured with high reproducibility, and enables the quantification of patient's sample comprising a high concentration of PSA, and preparation method therefor, standard solution for PSA quantification, and PSA quantification method. The standard substance comprises a compound having the structure of a PSA with a sugar chain represented by any of the following formulae A to D, and is isolated and purified from a natural product, chemically or enzymatically altered from a natural product, or the compound is artificially synthesized.