The present invention relates to a primer for PCR obtained by, directly or through inosine as a linker, linking a complementary nucleotide sequence or a complementary nucleotide sequence including a mis-matched nucleotide sequence to the 5′-terminal of a forward or reverse primer; and to a PCR method including a step of mixing a nucleic acid template in a PCR composition including the primer and then performing PCR on the mixture. The primer for PCR of the present invention includes a complementary nucleotide sequence or a mis-matched nucleotide sequence in a complementary nucleotide sequence, which is linked to the 5′-terminal thereof directly or via a linker, thereby lowering the sensitivity increase due to the increase in amplification products and reducing non-specifically occurring reactions in PCR.

The present disclosure provides a molecular marker linked to Fusarium wilt resistance gene in tomato, and a method for obtaining the molecular marker linked to Fusarium wilt resistance gene in tomato. The molecular marker according to the present disclosure has a high specificity, and can be used to identify the resistance to Fusarium oxysporum f. sp. Physiological race 3 quickly and improve the breeding efficiency of the seeds resistant to Fusarium oxysporum f. sp. Physiological race 3. The molecular marker linked to Fusarium wilt resistance gene in tomato according to the present disclosure is prepared simply and has a low production cost. The molecular marker according to the present disclosure can be used to identify the resistance to Fusarium oxysporum f. sp. Physiological race 3, screen the tomato single plant which is resistant to Fusarium oxysporum f. sp. Physiological race 3, and/or determine the purity of hybrid seeds derived from hybridization of the tomato which is resistant to Fusarium oxysporum f. sp. Physiological race 3 and the tomato which is susceptible to Fusarium oxysporum f. sp. Physiological race 3. The molecular marker according to the present disclosure can be also used to prepare a kit, which has the same uses as that of the molecular marker according to the present disclosure.

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

The present invention concerns reagents for the reversible protection of biological molecules. It relates in particular to compounds derived from azaisatoic anhydride and their uses for the protection of biological molecules, particularly enzymes, in order to block their activity. The invention also relates to the biological molecules protected in this manner and to the methods for making use of these reagents.

Provided are a composition for a polymerase reaction, containing a nucleic acid polymerase and a 2-methacryloyloxyethyl phosphorylcholine (MPC)-containing zwitterionic copolymer detergent, a tube for a polymerase reaction, and a kit for a polymerase reaction. The stability of the composition for a polymerase reaction can be improved and the reliability of the results of polymerase reaction such as nucleic acid polymerization or amplification can be improved.

Provided are a composition for a polymerase reaction, containing a nucleic acid polymerase and a 2-methacryloyloxyethyl phosphorylcholine (MPC)-containing zwitterionic copolymer detergent, a tube for a polymerase reaction, and a kit for a polymerase reaction. The stability of the composition for a polymerase reaction can be improved and the reliability of the results of polymerase reaction such as nucleic acid polymerization or amplification can be improved.

In an amplification reaction in a microfluidic apparatus, the reaction is carried out using starting substances tagged with fluorophore and quencher. The detection of reaction products occurs according to the disclosure by a separation of fluorophore and quencher occurring in the context of the amplification reaction. For the detection reaction, at least one energy-transferring substance is added and the evaluation occurs on the basis of the fluorescence emission of the fluorophores which occurs.

In an amplification reaction in a microfluidic apparatus, the reaction is carried out using starting substances tagged with fluorophore and quencher. The detection of reaction products occurs according to the disclosure by a separation of fluorophore and quencher occurring in the context of the amplification reaction. For the detection reaction, at least one energy-transferring substance is added and the evaluation occurs on the basis of the fluorescence emission of the fluorophores which occurs.

Improved compositions and methods for using modified non-retroviral reverse transcriptase to perform 3′ extension of a nucleic acid, employ non-templated deoxynucleotide addition to a single-stranded nucleic acid and/or synthesis of complementary DNA using non-complementary nucleic acids as primer and template (RNA- or DNA-templated DNA polymerase activity.